<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>2304-0106</journal-id>
<journal-title><![CDATA[Anales de la Academia de Ciencias de Cuba]]></journal-title>
<abbrev-journal-title><![CDATA[Anales de la ACC]]></abbrev-journal-title>
<issn>2304-0106</issn>
<publisher>
<publisher-name><![CDATA[Academia de Ciencias de Cuba]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S2304-01062023000100002</article-id>
<title-group>
<article-title xml:lang="es"><![CDATA[Evaluación de medios de transporte para el diagnóstico del SARS-CoV-2 a partir de muestras clínicas]]></article-title>
<article-title xml:lang="en"><![CDATA[Evaluation of transportation means for the diagnosis of SARS-CoV-2, using clinical samples]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Montalvo Villalba]]></surname>
<given-names><![CDATA[María Caridad]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Valdés Ramírez]]></surname>
<given-names><![CDATA[Odalys]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Menes Llerena]]></surname>
<given-names><![CDATA[Dunia]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Núñez Quiroz]]></surname>
<given-names><![CDATA[Lirialys]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Naranjo González]]></surname>
<given-names><![CDATA[Celine]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Figueredo Amador]]></surname>
<given-names><![CDATA[Claudia]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Fonseca Quintana]]></surname>
<given-names><![CDATA[Magilé]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Labrada Rosado]]></surname>
<given-names><![CDATA[Alexis]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Fernández Yero]]></surname>
<given-names><![CDATA[José Luis]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Rodríguez Lay]]></surname>
<given-names><![CDATA[Licel de los Ángeles]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Guzmán Tirado]]></surname>
<given-names><![CDATA[María Guadalupe]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[López Almaguer]]></surname>
<given-names><![CDATA[Yanaris]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,Instituto de Medicina Tropical Pedro Kourí (IPK)  ]]></institution>
<addr-line><![CDATA[ La Habana]]></addr-line>
<country>Cuba</country>
</aff>
<aff id="Af2">
<institution><![CDATA[,Centro Nacional de Biopreparados (BioCen)  ]]></institution>
<addr-line><![CDATA[ La Habana]]></addr-line>
<country>Cuba</country>
</aff>
<aff id="Af3">
<institution><![CDATA[,Grupo de las Industrias Biotecnológica y Farmacéutica de Cuba (BioCubaFarma)  ]]></institution>
<addr-line><![CDATA[ La Habana]]></addr-line>
<country>Cuba</country>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>04</month>
<year>2023</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>04</month>
<year>2023</year>
</pub-date>
<volume>13</volume>
<numero>1</numero>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://scielo.sld.cu/scielo.php?script=sci_arttext&amp;pid=S2304-01062023000100002&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://scielo.sld.cu/scielo.php?script=sci_abstract&amp;pid=S2304-01062023000100002&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://scielo.sld.cu/scielo.php?script=sci_pdf&amp;pid=S2304-01062023000100002&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="es"><p><![CDATA[RESUMEN  Introducción:  Para el diagnostico virológico de la infección por el coronavirus 2 del síndrome respiratorio agudo severo (SARS-CoV-2) y otras infecciones respiratorias virales ha sido necesaria la colecta de muestras clínicas en medio de transporte. Desde que se declaró el carácter pandémico de la COVID-19 y en aras de lograr soberanía tecnológica nacional para la detección del SARS-CoV-2, el Centro de Biopreparado produjo medios transportadores de exudado nasofaríngeos (ENF) que fueron evaluados en el Instituto de Medicina Tropical Pedro Kouri (IPK). El objetivo de la presente investigación fue evaluar el desempeño del medio de transporte universal (BTU) y el medio de transporte de ácidos nucleicos (TAN) para el diagnóstico por RT-PCR del SARS-CoV-2.  Métodos:  Se presentó la evaluación del desempeño de los medios BTU y TAN, a partir de ENF colectados para la detección del SARS-CoV-2 mediante la técnica de reacción en cadena de la polimerasa en tiempo real (RT-PCR). Como indicadores del desempeño se estimó la diferencia entre mediana de los Ct obtenidos por los medios en evaluación y el de referencia del medio de transporte viral (BTV). Además, se evaluó la concordancia, el coeficiente Kappa y la estabilidad de las muestras de ENF a las 48 y 72 horas (h) para el medio BTU y para el medio TAN esta evaluación se extendió hasta las 168 h.  Resultados y discusión: La concordancia fue casi perfecta con las muestras colectadas en medio TAN, a diferencia del medio BTU donde se identificó una concordancia moderada. La estabilidad de las muestras fue del 100 % a las 48 h para los 2 medios evaluados, decreciendo a 85,71 % para el medio BTU a las 72 h. Para las muestras colectadas en medio TAN la estabilidad se mantuvo hasta las 168 h (100 %). No se observó diferencias significativas en los valores de Ct entre los medios evaluados y el de referencia. En conclusión, el desempeño de los medios evaluados fue adecuado para la detección del SARS-CoV-2 por RT-PCR, comparable con el medio de referencia BTV. Recomendamos extender la evaluación de estos medios de transporte para el diagnóstico microbiológico de otros microorganismos.]]></p></abstract>
<abstract abstract-type="short" xml:lang="en"><p><![CDATA[ABSTRACT  Introduction: Transportation means are necessary to a proper collection of clinical samples for diagnosis of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) and other respiratory viral infections. In order to achieve technological sovereignty, since COVID-19 was declared a pandemic, the Cuban National Center for Bioproducts developed and manufactured transportation means for nasopharyngeal swab (NPS) samples that were evaluated at IPK.  Methods:  The performance of BTU and TAN media, using NPS for detection of SARS-CoV-2 was evaluated with RT-PCR. As indicators of performance, we estimated the difference between Ct values obtained with studied media and reference media (BTV). Moreover, the agreement, the Kappa coefficient, and the stability of NPS samples were evaluated at 48 and 72 hours (h) for BTU, while for TAN this evaluation was followed up to 168 h.  Results and discussion:  The agreement was almost perfect with samples collected in TAN medium, whereas BTU showed moderate agreement. The stability of samples was 100 % for both mediums at 48 h, decreasing to 85,71 % for BTU medium at 72 h. However, for samples collected in TAN medium the stability was 100 % till 168 h. In contrast, no significant difference was observed regarding Ct between evaluated and reference media. It is concluded that the performance of evaluated media was adequate for SARS-CoV-2 detection using RT-PCR, comparable with BTV reference media. Our recommendation is to extend the evaluation of these media for microbiological diagnosis of other microorganisms.]]></p></abstract>
<kwd-group>
<kwd lng="es"><![CDATA[medios de transporte de virus]]></kwd>
<kwd lng="es"><![CDATA[SARS-CoV-2, RT-PCR]]></kwd>
<kwd lng="en"><![CDATA[viral transport medium]]></kwd>
<kwd lng="en"><![CDATA[SARS-CoV-2, RT-PCR]]></kwd>
</kwd-group>
</article-meta>
</front><back>
<ref-list>
<ref id="B1">
<label>1</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Sood]]></surname>
<given-names><![CDATA[S]]></given-names>
</name>
<name>
<surname><![CDATA[Aggarwal]]></surname>
<given-names><![CDATA[V]]></given-names>
</name>
<name>
<surname><![CDATA[Aggarwal]]></surname>
<given-names><![CDATA[D]]></given-names>
</name>
<name>
<surname><![CDATA[Upadhyay]]></surname>
<given-names><![CDATA[SK]]></given-names>
</name>
<name>
<surname><![CDATA[Sak]]></surname>
<given-names><![CDATA[K]]></given-names>
</name>
<name>
<surname><![CDATA[Tuli]]></surname>
<given-names><![CDATA[HS]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[COVID-19 Pandemic: from Molecular Biology, Pathogenesis, Detection, and Treatment to Global Societal Impact]]></article-title>
<source><![CDATA[Curr Pharmacol Rep]]></source>
<year>2020</year>
<volume>6</volume>
<numero>5</numero>
<issue>5</issue>
<page-range>212-27</page-range></nlm-citation>
</ref>
<ref id="B2">
<label>2</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Martínez Díaz]]></surname>
<given-names><![CDATA[E]]></given-names>
</name>
<name>
<surname><![CDATA[Pérez Rodríguez]]></surname>
<given-names><![CDATA[R]]></given-names>
</name>
<name>
<surname><![CDATA[Herrera Martínez]]></surname>
<given-names><![CDATA[L]]></given-names>
</name>
<name>
<surname><![CDATA[Lage Dávila]]></surname>
<given-names><![CDATA[A]]></given-names>
</name>
<name>
<surname><![CDATA[Castellanos Serra]]></surname>
<given-names><![CDATA[L]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[La industria biofarmaceútica cubana en el combate contra la pandemia de COVID-19]]></article-title>
<source><![CDATA[An Acad Cienci Cuba]]></source>
<year>2020</year>
<volume>10</volume>
<numero>2</numero>
<issue>2</issue>
</nlm-citation>
</ref>
<ref id="B3">
<label>3</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Lobaina Rodríguez]]></surname>
<given-names><![CDATA[T]]></given-names>
</name>
<name>
<surname><![CDATA[De Armas Rodríguez]]></surname>
<given-names><![CDATA[J]]></given-names>
</name>
<name>
<surname><![CDATA[Rodríguez Cabrera]]></surname>
<given-names><![CDATA[T]]></given-names>
</name>
<name>
<surname><![CDATA[González García]]></surname>
<given-names><![CDATA[G]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Estrategia de ciencia, tecnología e innovación del Centro Nacional de Biopreparados frente a la COVID-19]]></article-title>
<source><![CDATA[An Acad Cienc Cuba]]></source>
<year>2020</year>
<volume>10</volume>
<numero>3</numero>
<issue>3</issue>
</nlm-citation>
</ref>
<ref id="B4">
<label>4</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Richard Greenblatt]]></surname>
<given-names><![CDATA[M]]></given-names>
</name>
<name>
<surname><![CDATA[Comar]]></surname>
<given-names><![CDATA[CE]]></given-names>
</name>
<name>
<surname><![CDATA[Flevaud]]></surname>
<given-names><![CDATA[L]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Copan eNAT Transport System To Address Challenges in COVID-19 Diagnostics in Regions with Limited Testing Access]]></article-title>
<source><![CDATA[J Clin Microbiol]]></source>
<year>2021</year>
<volume>59</volume>
<numero>5</numero>
<issue>5</issue>
</nlm-citation>
</ref>
<ref id="B5">
<label>5</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Montalvo Villalba]]></surname>
<given-names><![CDATA[MC]]></given-names>
</name>
<name>
<surname><![CDATA[Sosa Glaria]]></surname>
<given-names><![CDATA[E]]></given-names>
</name>
<name>
<surname><![CDATA[Rodríguez Lay]]></surname>
<given-names><![CDATA[LA]]></given-names>
</name>
<name>
<surname><![CDATA[Valdés]]></surname>
<given-names><![CDATA[O]]></given-names>
</name>
<name>
<surname><![CDATA[Vallina]]></surname>
<given-names><![CDATA[D]]></given-names>
</name>
<name>
<surname><![CDATA[Arencibia]]></surname>
<given-names><![CDATA[A]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Performance evaluation of Elecsys SARS-CoV-2 Antigen immunoassay for diagnostic of COVID-19]]></article-title>
<source><![CDATA[J Med Virol]]></source>
<year>2021</year>
<volume>94</volume>
<numero>3</numero>
<issue>3</issue>
<page-range>1001-8</page-range></nlm-citation>
</ref>
<ref id="B6">
<label>6</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Montalvo Villalba]]></surname>
<given-names><![CDATA[MC]]></given-names>
</name>
<name>
<surname><![CDATA[Labrada]]></surname>
<given-names><![CDATA[A]]></given-names>
</name>
<name>
<surname><![CDATA[Valdes]]></surname>
<given-names><![CDATA[O]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Inactivación del virus Zika con medio de transporte de ácidos nucleicos; potencialmente aplicable a muestras clínicas para el diagnóstico molecular de SARS-CoV-2]]></article-title>
<source><![CDATA[Rev Med Tropical]]></source>
<year>2022</year>
</nlm-citation>
</ref>
<ref id="B7">
<label>7</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[McHugh]]></surname>
<given-names><![CDATA[ML]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Interrater reliability: the kappa statistic]]></article-title>
<source><![CDATA[Biochem Med (Zagreb)]]></source>
<year>2012</year>
<volume>22</volume>
<numero>3</numero>
<issue>3</issue>
<page-range>276-82</page-range></nlm-citation>
</ref>
<ref id="B8">
<label>8</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Iglói]]></surname>
<given-names><![CDATA[Z]]></given-names>
</name>
<name>
<surname><![CDATA[Leven]]></surname>
<given-names><![CDATA[M]]></given-names>
</name>
<name>
<surname><![CDATA[Abdel Karem Abou Nouar]]></surname>
<given-names><![CDATA[Z]]></given-names>
</name>
<name>
<surname><![CDATA[Weller]]></surname>
<given-names><![CDATA[B]]></given-names>
</name>
<name>
<surname><![CDATA[Matheeussen]]></surname>
<given-names><![CDATA[V]]></given-names>
</name>
<name>
<surname><![CDATA[Coppens]]></surname>
<given-names><![CDATA[J]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Comparison of commercial real time reverse transcription PCR assays for the detection of SARS-CoV-2]]></article-title>
<source><![CDATA[J Clin Virol]]></source>
<year>2020</year>
<volume>129</volume>
</nlm-citation>
</ref>
<ref id="B9">
<label>9</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Welch]]></surname>
<given-names><![CDATA[SR]]></given-names>
</name>
<name>
<surname><![CDATA[Davies]]></surname>
<given-names><![CDATA[KA]]></given-names>
</name>
<name>
<surname><![CDATA[Buczkowski]]></surname>
<given-names><![CDATA[H]]></given-names>
</name>
<name>
<surname><![CDATA[Hettiarachchi]]></surname>
<given-names><![CDATA[N]]></given-names>
</name>
<name>
<surname><![CDATA[Green]]></surname>
<given-names><![CDATA[N]]></given-names>
</name>
<name>
<surname><![CDATA[Arnold]]></surname>
<given-names><![CDATA[U]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Analysis of Inactivation of SARS-CoV-2 by Specimen Transport Media, Nucleic Acid Extraction Reagents, Detergents, and Fixatives]]></article-title>
<source><![CDATA[J Clin Microbiol]]></source>
<year>2020</year>
<volume>58</volume>
<numero>11</numero>
<issue>11</issue>
</nlm-citation>
</ref>
<ref id="B10">
<label>10</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Blow]]></surname>
<given-names><![CDATA[JA]]></given-names>
</name>
<name>
<surname><![CDATA[Mores]]></surname>
<given-names><![CDATA[CN]]></given-names>
</name>
<name>
<surname><![CDATA[Dyer]]></surname>
<given-names><![CDATA[JD]]></given-names>
</name>
<name>
<surname><![CDATA[Dohm]]></surname>
<given-names><![CDATA[DJ]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Viral nucleic acid stabilization by RNA extraction reagent]]></article-title>
<source><![CDATA[J Virol Methods]]></source>
<year>2008</year>
<volume>150</volume>
<numero>1</numero>
<issue>1</issue>
<page-range>41-4</page-range></nlm-citation>
</ref>
<ref id="B11">
<label>11</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Luinstra]]></surname>
<given-names><![CDATA[K]]></given-names>
</name>
<name>
<surname><![CDATA[Petrich]]></surname>
<given-names><![CDATA[A]]></given-names>
</name>
<name>
<surname><![CDATA[Castriciano]]></surname>
<given-names><![CDATA[S]]></given-names>
</name>
<name>
<surname><![CDATA[Ackerman]]></surname>
<given-names><![CDATA[M]]></given-names>
</name>
<name>
<surname><![CDATA[Chong]]></surname>
<given-names><![CDATA[S]]></given-names>
</name>
<name>
<surname><![CDATA[Carruthers]]></surname>
<given-names><![CDATA[S]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Evaluation and clinical validation of an alcohol-based transport medium for preservation and inactivation of respiratory viruses]]></article-title>
<source><![CDATA[J Clin Microbiol]]></source>
<year>2011</year>
<volume>49</volume>
<numero>6</numero>
<issue>6</issue>
<page-range>2138-42</page-range></nlm-citation>
</ref>
</ref-list>
</back>
</article>
