<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>0253-570X</journal-id>
<journal-title><![CDATA[Revista de Salud Animal]]></journal-title>
<abbrev-journal-title><![CDATA[Rev Salud Anim.]]></abbrev-journal-title>
<issn>0253-570X</issn>
<publisher>
<publisher-name><![CDATA[Centro Nacional de Sanidad Agropecuaria]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S0253-570X2018000300007</article-id>
<title-group>
<article-title xml:lang="es"><![CDATA[Nuevas tendencias en el diagnóstico de enfermedades virales en los animales]]></article-title>
<article-title xml:lang="en"><![CDATA[New trends in the diagnosis of animal viral diseases]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Perera]]></surname>
<given-names><![CDATA[Carmen Laura]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Acevedo]]></surname>
<given-names><![CDATA[Ana María]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,Centro Nacional de Sanidad Agropecuaria Dirección de Salud Animal Grupo de Virología Animal]]></institution>
<addr-line><![CDATA[San José de las Lajas Mayabeque]]></addr-line>
<country>Cuba</country>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>12</month>
<year>2018</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>12</month>
<year>2018</year>
</pub-date>
<volume>40</volume>
<numero>3</numero>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://scielo.sld.cu/scielo.php?script=sci_arttext&amp;pid=S0253-570X2018000300007&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://scielo.sld.cu/scielo.php?script=sci_abstract&amp;pid=S0253-570X2018000300007&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://scielo.sld.cu/scielo.php?script=sci_pdf&amp;pid=S0253-570X2018000300007&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="es"><p><![CDATA[RESUMEN El diagnóstico y control de las enfermedades virales de importancia económica ha progresado de forma notable en la última década, gracias a la aplicación de las tecnologías de reacción en cadena de la polimerasa (PCR) y a las diferentes aplicaciones de la misma, como son la reacción de PCR basadas en la detección de fluorescencia en tiempo real (rPCR), la amplificación isotérmica mediada por bucle (LAMP) y las técnicas basadas en la secuenciación de ácidos nucleicos. Estos enfoques permiten la amplificación, la cuantificación simultánea y el análisis de secuencias de ácidos nucleicos, donde se combinan rapidez con elevadas sensibilidad y especificidad; sus beneficios con relación a los ensayos de PCR convencional o de punto final incluyen, además de un elevado rango dinámico, reducido riesgo de contaminación cruzada, capacidad para ser escalados y cuantificación precisa de la diana, lo que permite la determinación de la carga viral.]]></p></abstract>
<abstract abstract-type="short" xml:lang="en"><p><![CDATA[ABSTRACT The diagnosis and control of economically important viral diseases have progressed significantly in the last decade due to the application of polymerase chain reaction (PCR) technologies, as well as various applications thereof such as the PCR reaction based on the fluorescence detection in real time (rPCR), the loop-mediated isothermal amplification (LAMP) and the sequencing techniques based on nucleic acids. These approaches allow the amplification, simultaneous quantification and sequence analysis of nucleic acids, combining high speed with sensitivity and specificity, the benefits related to the conventional or endpoint PCR assays, a high dynamic range, a reduced cross contamination, the ability to be scaled, and the precise quantification of the target, allowing the determination of the viral load.]]></p></abstract>
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<kwd lng="es"><![CDATA[diagnóstico molecular]]></kwd>
<kwd lng="es"><![CDATA[PCR]]></kwd>
<kwd lng="es"><![CDATA[rPCR]]></kwd>
<kwd lng="es"><![CDATA[secuenciación]]></kwd>
<kwd lng="es"><![CDATA[LAMP]]></kwd>
<kwd lng="en"><![CDATA[molecular diagnosis]]></kwd>
<kwd lng="en"><![CDATA[PCR]]></kwd>
<kwd lng="en"><![CDATA[rPCR]]></kwd>
<kwd lng="en"><![CDATA[sequencing]]></kwd>
<kwd lng="en"><![CDATA[LAMP]]></kwd>
</kwd-group>
</article-meta>
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