<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>2074-8647</journal-id>
<journal-title><![CDATA[Biotecnología Vegetal]]></journal-title>
<abbrev-journal-title><![CDATA[Biot. Veg.]]></abbrev-journal-title>
<issn>2074-8647</issn>
<publisher>
<publisher-name><![CDATA[Instituto de Biotecnología de las PlantasUniversidad Central Marta Abreu de Las Villas]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S2074-86472020000400283</article-id>
<title-group>
<article-title xml:lang="en"><![CDATA[Callogenesis and rhizogenesis of Viola odorata L.]]></article-title>
<article-title xml:lang="es"><![CDATA[Callogénesis y rizogénesis de Viola odorata L.]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Vilas Haralkar]]></surname>
<given-names><![CDATA[Krupali]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Raosaheb Biradar]]></surname>
<given-names><![CDATA[Sanjay]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,Bharat Shikshan Sansthas Arts Science Commerce College Department of Botany ]]></institution>
<addr-line><![CDATA[Osmanabad (MS).]]></addr-line>
<country>India</country>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>12</month>
<year>2020</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>12</month>
<year>2020</year>
</pub-date>
<volume>20</volume>
<numero>4</numero>
<fpage>283</fpage>
<lpage>289</lpage>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://scielo.sld.cu/scielo.php?script=sci_arttext&amp;pid=S2074-86472020000400283&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://scielo.sld.cu/scielo.php?script=sci_abstract&amp;pid=S2074-86472020000400283&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://scielo.sld.cu/scielo.php?script=sci_pdf&amp;pid=S2074-86472020000400283&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="en"><p><![CDATA[ABSTRACT Viola odorata L. is a medicinal plant and source of different secondary metabolites. It has been proven that callus culture can be used as an effective method for multiplication of medicinal plants. The aim of this paper was to develop a protocol for callogenesis and rhizogenesis in V. odorata. Petiole and leaf were used as explants for callogenesis. Significant callus induction was observed on MS medium supplemented with 2 mg l-1 2,4-D + 2 mg l-1 NAA+30 mg l-1 adenine sulphate. It was produced hard and embryogenic callus with white and greenish colour. The highest fresh weight of callus was obtained with 2.5 mg l-1 2,4-D + 2.5 mg l-1 BAP (8.093 g) and the lowest with 0.5 mg l-1 BAP (0.295 g). Besides, the highest dry weight of callus was achieved with 2 mg l-1 2,4-D +2 mg l-1 NAA+30 mg l-1 adenine sulphate (0.755 g) and the lowest with 0.5 mg l-1 TDZ (0.045 g). Roots from callus were induced using IAA and IBA. Maximum number of roots (10.60) and highest root length (2.92 cm) were recorded after 15 to 20 days in culture medium with 1.5 mg l-1 IBA. Present study reveals that this protocol could be used for in vitro callogenesis and rhizogenesis of V. odorata.]]></p></abstract>
<abstract abstract-type="short" xml:lang="es"><p><![CDATA[RESUMEN Viola odorata L. es una planta medicinal y fuente de diferentes metabolitos secundarios. Se ha demostrado que el cultivo de callos se puede utilizar como un método eficaz para la multiplicación de plantas medicinales. El objetivo de este trabajo fue desarrollar un protocolo para desarrollar callogénesis y rizogénesis en V. odorata. Se utilizaron pecíolos y hojas como explantes para la callogénesis. Se observó una inducción significativa de callos en el medio de cultivo MS con 2 mg l-1 2,4-D + 2 mg l-1 NAA + 30 mg l-1 adenina sulfato. Se produjeron callos duros y embriogénicos con color blanco y verdoso. La masa fresca más elevada de callo se obtuvo con 2.5 mg l-1 2,4-D + 2.5 mg l-1 BAP (8.093 g) y la más baja con 0.5 mg l-1 BAP (0.295 g). Además, la masa seca más alta se obtuvo en medio de cultivo con 2 mg l-1 2,4-D + 2 mg l-1 NAA + 30 mg l-1 de sulfato de adenina (0.755 g) y la más baja con 0.5 mg l-1 TDZ (0.045 g). La inducción de raíces del callo se produjo con el uso de AIA e AIB. El número máximo de raíces y la mayor longitud de raíz (2.920 cm) se registró después de 15 a 20 días con 1.5 mg l-1 de AIB. El presente estudio revela que este protocolo podría utilizarse para la callogénesis y rizogénesis in vitro de V. odorata L.]]></p></abstract>
<kwd-group>
<kwd lng="en"><![CDATA[auxins]]></kwd>
<kwd lng="en"><![CDATA[callus]]></kwd>
<kwd lng="en"><![CDATA[cytokinins]]></kwd>
<kwd lng="en"><![CDATA[in vitro]]></kwd>
<kwd lng="es"><![CDATA[auxinas]]></kwd>
<kwd lng="es"><![CDATA[callo]]></kwd>
<kwd lng="es"><![CDATA[citoquininas]]></kwd>
<kwd lng="es"><![CDATA[in vitro]]></kwd>
</kwd-group>
</article-meta>
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