<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>2223-4861</journal-id>
<journal-title><![CDATA[Centro Azúcar]]></journal-title>
<abbrev-journal-title><![CDATA[cen. az.]]></abbrev-journal-title>
<issn>2223-4861</issn>
<publisher>
<publisher-name><![CDATA[Editorial Feijóo]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S2223-48612022000200001</article-id>
<title-group>
<article-title xml:lang="es"><![CDATA[OPTIMIZACIÓN DE PARÁMETROS OPERACIONALES, ESCALADO Y DETERMINACIÓN DE REQUERIMIENTOS PARA LA INVERSIÓN ENZIMÁTICA DE SACAROSA]]></article-title>
<article-title xml:lang="en"><![CDATA[OPTIMIZATION OF OPERATIONAL PARAMETERS, SCALE-UP AND DETERMINATION OF REQUIREMENTS FOR ENZYMATIC INVERSION OF SUCROSE]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Acosta Solares]]></surname>
<given-names><![CDATA[Amanda]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Pérez Navarro]]></surname>
<given-names><![CDATA[Omar]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Pérez Cruz]]></surname>
<given-names><![CDATA[Enrique R.]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Martínez García]]></surname>
<given-names><![CDATA[Duniesky]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,Universidad Central &#8220;Marta Abreu&#8221; de las Villas Facultad de Química y Farmacia Departamento de Ingeniería Química]]></institution>
<addr-line><![CDATA[Santa Clara Villa Clara]]></addr-line>
<country>Cuba</country>
</aff>
<aff id="Af2">
<institution><![CDATA[,Centro de Ingeniería Genética y Biotecnología de Sancti Spíritus (CIGBSS) Laboratorio de Fermentaciones ]]></institution>
<addr-line><![CDATA[ Sancti Spíritus]]></addr-line>
<country>Cuba</country>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>06</month>
<year>2022</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>06</month>
<year>2022</year>
</pub-date>
<volume>49</volume>
<numero>2</numero>
<fpage>1</fpage>
<lpage>14</lpage>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://scielo.sld.cu/scielo.php?script=sci_arttext&amp;pid=S2223-48612022000200001&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://scielo.sld.cu/scielo.php?script=sci_abstract&amp;pid=S2223-48612022000200001&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://scielo.sld.cu/scielo.php?script=sci_pdf&amp;pid=S2223-48612022000200001&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="es"><p><![CDATA[RESUMEN  Introducción: Una propuesta atractiva y novedosa para la aplicación de la inversión enzimática de la sacarosa a escala industrial, lo constituye la &#946;-fructosidasa de Thermotoga maritima (BfrA) expresada en Pichia pastoris e inmovilizada en alginato de calcio (PpABfrA), dada la elevada termoestabilidad y termoactividad de este biocatalizador.  Objetivo: Determinar las condiciones operacionales para el escalado industrial de la hidrólisis enzimática de la sacarosa en una instalación productora de glucosa, a partir de las variables experimentales óptimas y los requerimientos de facilidades auxiliares.  Materiales y Métodos: Se combinó el diseño de experimentos y la optimización del comportamiento de las variables experimentales influyentes en el porcentaje de hidrólisis alcanzado con el biocatalizador termoestable PpABfrA. Se escalaron las condiciones reactivas experimentales y se seleccionó la tecnología para la inserción de este método, con los consumos correspondientes.  Resultados y Discusión: El tiempo óptimo de reacción resultó ser 12 h para una concentración de sacarosa de 1,75 mol/l, donde se alcanza un 85,6 % de inversión. A su vez, mantener el cizallamiento del biocatalizador constante en el reactor se seleccionó como criterio de escalado más acertado. Con la concepción tecnológica de esta etapa se estimó una capacidad de hidrólisis de 201,6 t de sacarosa en 11 días donde se realizan 16 ciclos de inversiones con 2,10 t de biocatalizador, en un volumen efectivo de reacción de 24 m3.  Conclusiones: Los parámetros operacionales determinados a escala industrial para la inserción de esta tecnología, aseguran el incremento del rendimiento y calidad de la glucosa obtenida.]]></p></abstract>
<abstract abstract-type="short" xml:lang="en"><p><![CDATA[ABSTRACT  Introduction: An attractive and novel proposal for the application of enzymatic inversion of sucrose on industrial scale is constituted by &#946;-fructosidase from Thermotoga maritima (BfrA) expressed in Pichia pastoris and immobilized on calcium alginate (PpABfrA), because the high thermostability and thermoactivity of this biocatalyst.  Objective: To determine the operational conditions for the industrial scale-up of enzymatic hydrolysis of sucrose in a glucose production facility, based on the optimal experimental variables and the requirements for auxiliary facilities.  Materials and Methods: Design of experiments and optimization of the behavior of the experimental variables influencing the percentage of hydrolysis achieved with the thermostable biocatalyst PpABfrA were combined. The experimental reactive conditions were scaled and the technology for this inversion method insertion was selected, with its corresponding consumptions.  Results and Discussion: The optimum reaction time was 12 h for a sucrose concentration of 1.75 M, where 85.6 % inversion is achieved. In turn, keeping the biocatalyst shear constant in the reactor was selected as the most successful scaling criterion. With the technological conception of this stage, a hydrolysis capacity of 201.6 t of sucrose in 11 days was estimated, where 16 inversion cycles are carried out with 2.10 t of biocatalyst, in an effective reaction volume of 24 m3.  Conclusions: The operational parameters determined on industrial scale for the insertion of this technology, ensure an increase the yield and quality of obtained glucose.]]></p></abstract>
<kwd-group>
<kwd lng="es"><![CDATA[biocatalizador]]></kwd>
<kwd lng="es"><![CDATA[escalado]]></kwd>
<kwd lng="es"><![CDATA[glucosa]]></kwd>
<kwd lng="es"><![CDATA[hidrólisis enzimática]]></kwd>
<kwd lng="es"><![CDATA[óptimos, termoestabilidad]]></kwd>
<kwd lng="en"><![CDATA[biocatalyst]]></kwd>
<kwd lng="en"><![CDATA[scale-up]]></kwd>
<kwd lng="en"><![CDATA[glucose]]></kwd>
<kwd lng="en"><![CDATA[enzymatic hydrolysis]]></kwd>
<kwd lng="en"><![CDATA[optimums]]></kwd>
<kwd lng="en"><![CDATA[thermostability]]></kwd>
</kwd-group>
</article-meta>
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