<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>2224-7920</journal-id>
<journal-title><![CDATA[Revista de Producción Animal]]></journal-title>
<abbrev-journal-title><![CDATA[Rev. prod. anim.]]></abbrev-journal-title>
<issn>2224-7920</issn>
<publisher>
<publisher-name><![CDATA[Ediciones Universidad de Camagüey]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S2224-79202019000100037</article-id>
<title-group>
<article-title xml:lang="es"><![CDATA[Centrifugación coloidal como método de selección espermática previo a la crioconservación con glicerol en caninos]]></article-title>
<article-title xml:lang="en"><![CDATA[Colloidal Centrifugation as a Method of Sperm Selection Prior to Cryopreservation with Glycerol in Canines]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Ayala Guanga]]></surname>
<given-names><![CDATA[Luis]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Garay Peña]]></surname>
<given-names><![CDATA[Gabriela]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Carpio Alemán]]></surname>
<given-names><![CDATA[Fredy]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Rodas Carpio]]></surname>
<given-names><![CDATA[Ramiro]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Nieto Escandón]]></surname>
<given-names><![CDATA[Pedro]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Calle Ortiz]]></surname>
<given-names><![CDATA[Guido]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,Universidad de Cuenca Facultad de Ciencias Agropecuarias ]]></institution>
<addr-line><![CDATA[ ]]></addr-line>
<country>Ecuador</country>
</aff>
<aff id="Af2">
<institution><![CDATA[,Universidad de Cuenca Facultad de Ciencias Agropecuarias ]]></institution>
<addr-line><![CDATA[ ]]></addr-line>
<country>Ecuador</country>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>04</month>
<year>2019</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>04</month>
<year>2019</year>
</pub-date>
<volume>31</volume>
<numero>1</numero>
<fpage>37</fpage>
<lpage>46</lpage>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://scielo.sld.cu/scielo.php?script=sci_arttext&amp;pid=S2224-79202019000100037&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://scielo.sld.cu/scielo.php?script=sci_abstract&amp;pid=S2224-79202019000100037&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://scielo.sld.cu/scielo.php?script=sci_pdf&amp;pid=S2224-79202019000100037&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="es"><p><![CDATA[Resumen  Antecedentes: El eyaculado del perro presenta tres fracciones, la primera y tercera que contienen líquido prostático que es perjudicial para la criopreservación, y la segunda rica en espermatozoides. Obtener únicamente la segunda fracción del eyaculado es complicado; por lo tanto, se evaluó la centrifugación coloidal como método de selección espermática, más la adición de glicerol en tres concentraciones 4; 6 y 8 % como crio protector.  Métodos: El estudio fue realizado en la Universidad de Cuenca-Ecuador, se valoraron 72 muestras seminales procedentes de 6 perros mestizos. Cada muestra fue dividida en 2 alícuotas, la primera para el Grupo 1 (centrifugación convencional) y la otra Grupo 2 (centrifugación coloidal). Una vez centrifugadas cada alícuota fue sub-dividida en tres y se les adicionó 4; 6 y 8 % de glicerol. Se realizaron 3 evaluaciones espermáticas (inicial, pre y post descongelación).  Resultados: La concentración espermática luego de la centrifugación fue similar entre la muestra inicial (MI) y Grupo 1 (P &gt; 0,05); en Grupo 2 se observó menos espermatozoides (P &lt; 0,05). Al valorar la motilidad individual progresiva (MIP) pre congelación, se observó que los espermatozoides del Grupo 1 redujeron un 30 % su motilidad en relación a la muestra MI y la de Grupo 2. La MIP post descongelación dentro de los grupos con diferentes concentraciones de glicerol (4; 6 y 8 %), que recibieron previamente centrifugación con Percoll (Grupo 2) presentaron diferencia estadística (P &lt; 0,05). Sin embargo, la prueba de HOS proporcionó porcentajes similares.  Conclusiones:  Se concluye que la centrifugación coloidal, previa al proceso de congelación es una técnica que permite mayor selección y purificación espermática.]]></p></abstract>
<abstract abstract-type="short" xml:lang="en"><p><![CDATA[Abstract  Background:  The ejaculate of the dog has three fractions, the first and third containing prostatic fluid that is harmful to cryopreservation, and the second rich in sperm. Obtaining only the second fraction of the ejaculate is complicated; therefore, colloidal centrifugation was evaluated as a sperm selection method, plus the addition of glycerol in three concentrations 4, 6 and 8 % as cryoprotector.  Methods:  The study was conducted at the University of Cuenca-Ecuador, 72 seminal samples from 6 crossbreed dogs were evaluated. Each sample was divided into 2 aliquots, the first for Group 1 (conventional centrifugation) and the other Group 2 (colloidal centrifugation), once centrifuged each aliquot was sub-divided into three and 4, 6 and 8 % of glycerol were added. Three spermatic evaluations were carried out (initial, pre and post thawing).  Results:  The sperm concentration after centrifugation was similar between the initial sample (MI) and Group 1 (P &gt; 0.05); in Group 2 less sperm was observed (P &lt; 0.05). When assessing the progressive individual motility (MIP) pre freezing, it was observed that the sperm of the Group 1 reduced 30 % its motility in relation to the sample MI and that of Group 2. Post thawing MIP within the groups with different concentrations of glycerol (4, 6, 8 %), which previously received centrifugation with Percoll (Group 2) presented statistical difference (P &lt; 0.05). However, the HOS test gave similar percentages.  Conclusions: It is concluded that colloidal centrifugation, prior to the freezing process, is a technique that allows greater selection and sperm purification.]]></p></abstract>
<kwd-group>
<kwd lng="es"><![CDATA[centrifugación coloidal]]></kwd>
<kwd lng="es"><![CDATA[semen]]></kwd>
<kwd lng="es"><![CDATA[perros]]></kwd>
<kwd lng="es"><![CDATA[glicerol]]></kwd>
<kwd lng="en"><![CDATA[colloidal centrifugation]]></kwd>
<kwd lng="en"><![CDATA[semen]]></kwd>
<kwd lng="en"><![CDATA[dogs]]></kwd>
<kwd lng="en"><![CDATA[glycerol]]></kwd>
</kwd-group>
</article-meta>
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