<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>2304-0106</journal-id>
<journal-title><![CDATA[Anales de la Academia de Ciencias de Cuba]]></journal-title>
<abbrev-journal-title><![CDATA[Anales de la ACC]]></abbrev-journal-title>
<issn>2304-0106</issn>
<publisher>
<publisher-name><![CDATA[Academia de Ciencias de Cuba]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S2304-01062022000300007</article-id>
<title-group>
<article-title xml:lang="es"><![CDATA[Evento de modificación de soya transgénica para mayor resistencia a la roya asiática]]></article-title>
<article-title xml:lang="en"><![CDATA[Modification event of transgenic soybean that confers resistance to asian rust]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Soto Pérez]]></surname>
<given-names><![CDATA[Natacha]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Enríquez Obregón]]></surname>
<given-names><![CDATA[Gil Alberto]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Hernández Avera]]></surname>
<given-names><![CDATA[Yuniet]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Delgado Abad]]></surname>
<given-names><![CDATA[Celia]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Rosabal Ayan]]></surname>
<given-names><![CDATA[Yamilka]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Ortiz Pérez]]></surname>
<given-names><![CDATA[Rodobaldo]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Pujol Ferrer]]></surname>
<given-names><![CDATA[Merardo]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Ferreira Fabré]]></surname>
<given-names><![CDATA[Aleines]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Hernández Velázquez]]></surname>
<given-names><![CDATA[Abel]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,Centro de Ingeniería Genética y Biotecnología (CIGB)  ]]></institution>
<addr-line><![CDATA[ La Habana]]></addr-line>
<country>Cuba</country>
</aff>
<aff id="Af2">
<institution><![CDATA[,Instituto Nacional de Ciencias Agrícolas  ]]></institution>
<addr-line><![CDATA[ Mayabeque]]></addr-line>
<country>Cuba</country>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>12</month>
<year>2022</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>12</month>
<year>2022</year>
</pub-date>
<volume>12</volume>
<numero>3</numero>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://scielo.sld.cu/scielo.php?script=sci_arttext&amp;pid=S2304-01062022000300007&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://scielo.sld.cu/scielo.php?script=sci_abstract&amp;pid=S2304-01062022000300007&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://scielo.sld.cu/scielo.php?script=sci_pdf&amp;pid=S2304-01062022000300007&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="es"><p><![CDATA[RESUMEN  Introducción.  La roya asiática causada por el hongo Phakopsora pachyrhizi es la enfermedad más destructiva que afecta a la soya. El desarrollo de plantas transgénicas que portan defensinas ha ofrecido una alternativa para proteger al cultivo frente a hongos patógenos. Las defensinas son pequeños péptidos antimicrobianos presentes en las plantas, que han desempeñado un rol en su mecanismo de defensa natural.  Métodos.  Se transformaron ejes embriogénicos de soya con el gen de defensina NmDef02 aislado de Nicotiana megalosiphon mediante una metodología de transformación por balística optimizada que selecciona brotes resistentes al herbicida glifosato. Los clones transgénicos se analizaron molecularmente. Se evaluó la nodulación en plantas transgénicas inoculadas con la bacteria fijadora de nitrógeno Bradyrhizobium japonicum. Los clones transgénicos se evaluaron en campo frente a la infección natural con P. pachyrhizi, con licencia del Centro Nacional de Seguridad Biológica.  Resultados.  Se obtuvieron clones transgénicos resistentes a glifosato con elevada eficiencia de transformación. Se confirmó la integración y expresión del transgen mediante PCR, Southern blot y qRT-PCR. Se logró la nodulación en raíces de plantas transgénicas inoculadas con la bacteria B. japonicum. La expresión constitutiva del gen NmDef02 en los clones transgénicos indujo una elevada resistencia a P. pachyrhizi en campo, siendo superior en las plantas del clon Def1.  Conclusiones.  Por vez primera se transformaron plantas de soya con defensina, mediante una metodología de transformación adaptada a genotipos cubanos. La expresión del transcripto en plantas de soya no afectó la nodulación por B. japonicum, fundamental para la fijación de nitrógeno atmosférico. Ha constituido el primer reporte de resistencia a la roya asiática en plantas transgénicas de soya que expresan la defensina NmDef02.]]></p></abstract>
<abstract abstract-type="short" xml:lang="en"><p><![CDATA[ABSTRACT  Introduction. Asian rust caused by the Phakopsorapachyrhizi fungus is the most destructive disease affecting soybeans. The development of transgenic plants that carry defensins offers an alternative to protect the crop. Defensins are small antimicrobial peptides present in plants that play a role in their natural defense mechanism.  Methods. Soybean embryogenic axes were transformed with the NmDef02 defensin gene isolated from Nicotianamegalosiphon, using an optimized ballistics transformation methodology that selects shoots resistant to the herbicide glyphosate. The transgenic clones were analyzed molecularly. Nodulation was evaluated in transgenic plants inoculated with the nitrogen-fixing bacterium Bradyrhizobiumjaponicum. The transgenic clones were evaluated in the field against natural infection with P. pachyrhizi, licensed by the National Center for Biological Safety.  Results and Discussion. Glyphosate resistant transgenic clones with high transformation efficiency were obtained. Integration and expression of the transgene was confirmed by PCR, Southern blot and qRT-PCR. Efficient nodulation was achieved in roots of transgenic plants inoculated with B. japonicum bacterium. The severity of rust, fungal biomass and defoliation in plants were significantly lower in the transgenic clones. The constitutive expression of the NmDef02 gene in the transgenic clones induced a high resistance to P. pachyrhizi in the field, being higher in plants of the clone Def1. It is concluded that it is the first time that soybean plants have been transformed with a defensin, using a transformation methodology adapted to Cuban genotypes. The expression of the transcript in soybean plants does not affect nodulation by B. japonicum, which is essential for atmospheric nitrogen fixation. It is the first report of resistance to Asian rust in transgenic soybean plants that express the defensin NmDef02.]]></p></abstract>
<kwd-group>
<kwd lng="es"><![CDATA[defensina]]></kwd>
<kwd lng="es"><![CDATA[Phakopsora pachyrhizi]]></kwd>
<kwd lng="es"><![CDATA[soya]]></kwd>
<kwd lng="es"><![CDATA[resistencia]]></kwd>
<kwd lng="en"><![CDATA[defensin]]></kwd>
<kwd lng="en"><![CDATA[Phakopsora pachyrhizi]]></kwd>
<kwd lng="en"><![CDATA[soybean]]></kwd>
<kwd lng="en"><![CDATA[resistance]]></kwd>
</kwd-group>
</article-meta>
</front><back>
<ref-list>
<ref id="B1">
<label>1</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Menéndez]]></surname>
<given-names><![CDATA[C]]></given-names>
</name>
<name>
<surname><![CDATA[Trujillo]]></surname>
<given-names><![CDATA[LE]]></given-names>
</name>
<name>
<surname><![CDATA[Ramírez]]></surname>
<given-names><![CDATA[R]]></given-names>
</name>
<name>
<surname><![CDATA[González-Peña]]></surname>
<given-names><![CDATA[D]]></given-names>
</name>
<name>
<surname><![CDATA[Espinosa]]></surname>
<given-names><![CDATA[D]]></given-names>
</name>
<name>
<surname><![CDATA[Enriquez]]></surname>
<given-names><![CDATA[GA]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Producción de un inoculante líquido de Bradyrhizobium japonicum con alto impacto en la siembra mecanizada de la soya en Cuba]]></article-title>
<source><![CDATA[Biotecnología Aplicada]]></source>
<year>2014</year>
<volume>31</volume>
<numero>2</numero>
<issue>2</issue>
<page-range>116-20</page-range></nlm-citation>
</ref>
<ref id="B2">
<label>2</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Hartman]]></surname>
<given-names><![CDATA[GL]]></given-names>
</name>
<name>
<surname><![CDATA[Miles]]></surname>
<given-names><![CDATA[MR]]></given-names>
</name>
<name>
<surname><![CDATA[Frederick]]></surname>
<given-names><![CDATA[RD]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Breeding for resistance to soybean rust]]></article-title>
<source><![CDATA[Plant disease]]></source>
<year>2005</year>
<volume>89</volume>
<numero>6</numero>
<issue>6</issue>
<page-range>664-6</page-range></nlm-citation>
</ref>
<ref id="B3">
<label>3</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Tremblay]]></surname>
<given-names><![CDATA[A]]></given-names>
</name>
<name>
<surname><![CDATA[Hosseini]]></surname>
<given-names><![CDATA[P]]></given-names>
</name>
<name>
<surname><![CDATA[Li]]></surname>
<given-names><![CDATA[S]]></given-names>
</name>
<name>
<surname><![CDATA[Alkharouf]]></surname>
<given-names><![CDATA[NW]]></given-names>
</name>
<name>
<surname><![CDATA[Matthews]]></surname>
<given-names><![CDATA[BF]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Identification of genes expressed by Phakopsora pachyrhizi, the pathogen causing soybean rust, at a late stage of infection of susceptible soybean leaves]]></article-title>
<source><![CDATA[Plant Pathology]]></source>
<year>2012</year>
<volume>61</volume>
<page-range>773-86</page-range></nlm-citation>
</ref>
<ref id="B4">
<label>4</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Pérez]]></surname>
<given-names><![CDATA[L]]></given-names>
</name>
<name>
<surname><![CDATA[Martínez]]></surname>
<given-names><![CDATA[E]]></given-names>
</name>
<name>
<surname><![CDATA[Pérez]]></surname>
<given-names><![CDATA[M]]></given-names>
</name>
<name>
<surname><![CDATA[Martín]]></surname>
<given-names><![CDATA[EL]]></given-names>
</name>
<name>
<surname><![CDATA[Borrás]]></surname>
<given-names><![CDATA[HO]]></given-names>
</name>
<name>
<surname><![CDATA[Hernández]]></surname>
<given-names><![CDATA[I]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[First Report of Asian rust of soybean caused by Phakopsora pachyrhizi in Cuba]]></article-title>
<source><![CDATA[Plant Pathology]]></source>
<year>2010</year>
<volume>59</volume>
<numero>4</numero>
<issue>4</issue>
</nlm-citation>
</ref>
<ref id="B5">
<label>5</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Tavares]]></surname>
<given-names><![CDATA[LS]]></given-names>
</name>
<name>
<surname><![CDATA[Santos]]></surname>
<given-names><![CDATA[MO]]></given-names>
</name>
<name>
<surname><![CDATA[Viccini]]></surname>
<given-names><![CDATA[LF]]></given-names>
</name>
<name>
<surname><![CDATA[Moreira]]></surname>
<given-names><![CDATA[JS]]></given-names>
</name>
<name>
<surname><![CDATA[Miller]]></surname>
<given-names><![CDATA[RNG]]></given-names>
</name>
<name>
<surname><![CDATA[Franco]]></surname>
<given-names><![CDATA[OL]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Biotechnological potential of antimicrobial peptides from flowers]]></article-title>
<source><![CDATA[Peptides]]></source>
<year>2008</year>
<volume>29</volume>
<page-range>1842-51</page-range></nlm-citation>
</ref>
<ref id="B6">
<label>6</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Thomma]]></surname>
<given-names><![CDATA[BPHJ]]></given-names>
</name>
<name>
<surname><![CDATA[Cammue]]></surname>
<given-names><![CDATA[BPA]]></given-names>
</name>
<name>
<surname><![CDATA[Thevissen]]></surname>
<given-names><![CDATA[K]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Plant defensins]]></article-title>
<source><![CDATA[Planta]]></source>
<year>2002</year>
<volume>216</volume>
<page-range>193-202</page-range></nlm-citation>
</ref>
<ref id="B7">
<label>7</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Portieles]]></surname>
<given-names><![CDATA[R]]></given-names>
</name>
<name>
<surname><![CDATA[Ayra]]></surname>
<given-names><![CDATA[C]]></given-names>
</name>
<name>
<surname><![CDATA[Gonzalez]]></surname>
<given-names><![CDATA[E]]></given-names>
</name>
<name>
<surname><![CDATA[Gallo]]></surname>
<given-names><![CDATA[A]]></given-names>
</name>
<name>
<surname><![CDATA[Rodriguez]]></surname>
<given-names><![CDATA[R]]></given-names>
</name>
<name>
<surname><![CDATA[Chacón]]></surname>
<given-names><![CDATA[O]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[NmDef02, a novel antimicrobial gene isolated from Nicotiana megalosiphon confers high-level pathogen resistance under greenhouse and field conditions]]></article-title>
<source><![CDATA[Plant Biotechnology Journal]]></source>
<year>2010</year>
<volume>8</volume>
<numero>6</numero>
<issue>6</issue>
<page-range>678-90</page-range></nlm-citation>
</ref>
<ref id="B8">
<label>8</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Espinosa Huerta]]></surname>
<given-names><![CDATA[E]]></given-names>
</name>
<name>
<surname><![CDATA[Quintero Jiménez]]></surname>
<given-names><![CDATA[A]]></given-names>
</name>
<name>
<surname><![CDATA[Sánchez García]]></surname>
<given-names><![CDATA[BM]]></given-names>
</name>
<name>
<surname><![CDATA[Acosta Gallegos]]></surname>
<given-names><![CDATA[JA]]></given-names>
</name>
<name>
<surname><![CDATA[Mora-Avilés]]></surname>
<given-names><![CDATA[MA]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Resistencia a Colletotrichum lindemuthianum en frijol común transgénico, expresando el gen defensina de Arabidopsis thaliana]]></article-title>
<source><![CDATA[Revista Mexicana de Ciencias Agrícolas]]></source>
<year>2013</year>
<volume>4</volume>
<numero>7</numero>
<issue>7</issue>
<page-range>1027-42</page-range></nlm-citation>
</ref>
<ref id="B9">
<label>9</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Soto]]></surname>
<given-names><![CDATA[N]]></given-names>
</name>
<name>
<surname><![CDATA[Delgado]]></surname>
<given-names><![CDATA[C]]></given-names>
</name>
<name>
<surname><![CDATA[Hernández]]></surname>
<given-names><![CDATA[Y]]></given-names>
</name>
<name>
<surname><![CDATA[Rosabal]]></surname>
<given-names><![CDATA[Y]]></given-names>
</name>
<name>
<surname><![CDATA[Ferreira]]></surname>
<given-names><![CDATA[A]]></given-names>
</name>
<name>
<surname><![CDATA[Pujol]]></surname>
<given-names><![CDATA[M]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Efficient particle bombardment-mediated transformation of Cuban soybean (INCASoy-36) using glyphosate as a selective agent]]></article-title>
<source><![CDATA[Plant Cell, Tissue and Organ Culture (PCTOC)]]></source>
<year>2017</year>
<volume>128</volume>
<numero>1</numero>
<issue>1</issue>
<page-range>187-96</page-range></nlm-citation>
</ref>
<ref id="B10">
<label>10</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Soto]]></surname>
<given-names><![CDATA[N]]></given-names>
</name>
<name>
<surname><![CDATA[Hernández]]></surname>
<given-names><![CDATA[Y]]></given-names>
</name>
<name>
<surname><![CDATA[Delgado]]></surname>
<given-names><![CDATA[C]]></given-names>
</name>
<name>
<surname><![CDATA[Rosabal]]></surname>
<given-names><![CDATA[Y]]></given-names>
</name>
<name>
<surname><![CDATA[Ortiz]]></surname>
<given-names><![CDATA[R]]></given-names>
</name>
<name>
<surname><![CDATA[Valencia]]></surname>
<given-names><![CDATA[L]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Field resistance to Phakopsora pachyrhizi and Colletotrichum truncatum of transgenic soybean expressing the NmDef02 plant defensin gene]]></article-title>
<source><![CDATA[Frontiers in Plant Science]]></source>
<year>2020</year>
<volume>11</volume>
<page-range>562</page-range></nlm-citation>
</ref>
<ref id="B11">
<label>11</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Doyle]]></surname>
<given-names><![CDATA[J]]></given-names>
</name>
<name>
<surname><![CDATA[Doyle]]></surname>
<given-names><![CDATA[J]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[A rapid DNA isolation procedure for small quantities of fresh leaf tissue]]></article-title>
<source><![CDATA[Phytochemical Bulletin]]></source>
<year>1987</year>
<volume>19</volume>
<page-range>11-5</page-range></nlm-citation>
</ref>
<ref id="B12">
<label>12</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Muller]]></surname>
<given-names><![CDATA[P]]></given-names>
</name>
<name>
<surname><![CDATA[Janovjak]]></surname>
<given-names><![CDATA[H]]></given-names>
</name>
<name>
<surname><![CDATA[Miserez]]></surname>
<given-names><![CDATA[AR]]></given-names>
</name>
<name>
<surname><![CDATA[Dobbie]]></surname>
<given-names><![CDATA[Z]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Processing of gene expression data generated by quantitative real-time RT-PCR]]></article-title>
<source><![CDATA[Biotechniques]]></source>
<year>2002</year>
<volume>32</volume>
<page-range>1372-9</page-range></nlm-citation>
</ref>
<ref id="B13">
<label>13</label><nlm-citation citation-type="book">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Sambrook]]></surname>
<given-names><![CDATA[J]]></given-names>
</name>
<name>
<surname><![CDATA[Fritsch]]></surname>
<given-names><![CDATA[E]]></given-names>
</name>
<name>
<surname><![CDATA[Maniatis]]></surname>
<given-names><![CDATA[T]]></given-names>
</name>
</person-group>
<source><![CDATA[Molecular cloning: A laboratory manual]]></source>
<year>1989</year>
<edition>Segunda</edition>
<publisher-loc><![CDATA[Cold Spring Harbor, NY ]]></publisher-loc>
<publisher-name><![CDATA[Cold Spring Harbor Laboratory Press]]></publisher-name>
</nlm-citation>
</ref>
<ref id="B14">
<label>14</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Ploper]]></surname>
<given-names><![CDATA[LD]]></given-names>
</name>
<name>
<surname><![CDATA[Escobar]]></surname>
<given-names><![CDATA[D]]></given-names>
</name>
<name>
<surname><![CDATA[Ivancovich]]></surname>
<given-names><![CDATA[A]]></given-names>
</name>
<name>
<surname><![CDATA[Diaz]]></surname>
<given-names><![CDATA[CG]]></given-names>
</name>
<name>
<surname><![CDATA[Sillon]]></surname>
<given-names><![CDATA[M]]></given-names>
</name>
<name>
<surname><![CDATA[Formento]]></surname>
<given-names><![CDATA[N]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Propuesta de protocolo para muestreo y evaluación de la roya asiática de la soja en Argentina]]></article-title>
<source><![CDATA[Avance agroindustrial-Estación Experimental Agro-Industrial Obispo Colombres]]></source>
<year>2006</year>
<volume>27</volume>
<numero>3</numero>
<issue>3</issue>
<page-range>35-7</page-range></nlm-citation>
</ref>
<ref id="B15">
<label>15</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Nápoles García]]></surname>
<given-names><![CDATA[MC]]></given-names>
</name>
<name>
<surname><![CDATA[González-Anta]]></surname>
<given-names><![CDATA[G]]></given-names>
</name>
<name>
<surname><![CDATA[Ferreira]]></surname>
<given-names><![CDATA[A]]></given-names>
</name>
<name>
<surname><![CDATA[Rossi]]></surname>
<given-names><![CDATA[A]]></given-names>
</name>
<name>
<surname><![CDATA[Hernández Forte]]></surname>
<given-names><![CDATA[I]]></given-names>
</name>
<name>
<surname><![CDATA[Costales Menéndez]]></surname>
<given-names><![CDATA[D]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Efecto de diferentes inoculantes sobre la nodulación de la soya cultivada en condiciones de estrés]]></article-title>
<source><![CDATA[Cultivos Tropicales]]></source>
<year>2014</year>
<volume>35</volume>
<numero>4</numero>
<issue>4</issue>
<page-range>45-51</page-range></nlm-citation>
</ref>
<ref id="B16">
<label>16</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Zhang]]></surname>
<given-names><![CDATA[Z]]></given-names>
</name>
<name>
<surname><![CDATA[Zhao]]></surname>
<given-names><![CDATA[J]]></given-names>
</name>
<name>
<surname><![CDATA[Ding]]></surname>
<given-names><![CDATA[L]]></given-names>
</name>
<name>
<surname><![CDATA[Zou]]></surname>
<given-names><![CDATA[L]]></given-names>
</name>
<name>
<surname><![CDATA[Li]]></surname>
<given-names><![CDATA[Y]]></given-names>
</name>
<name>
<surname><![CDATA[Chen]]></surname>
<given-names><![CDATA[G]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Constitutive expression of a novel antimicrobial protein, Hcm1, confers resistance to both Verticillium and Fusarium wilts in cotton]]></article-title>
<source><![CDATA[Scientific Reports]]></source>
<year>2016</year>
<volume>6</volume>
</nlm-citation>
</ref>
<ref id="B17">
<label>17</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Yamanaka]]></surname>
<given-names><![CDATA[N]]></given-names>
</name>
<name>
<surname><![CDATA[Yamaoka]]></surname>
<given-names><![CDATA[Y]]></given-names>
</name>
<name>
<surname><![CDATA[Kato]]></surname>
<given-names><![CDATA[M]]></given-names>
</name>
<name>
<surname><![CDATA[Lemos]]></surname>
<given-names><![CDATA[NG]]></given-names>
</name>
<name>
<surname><![CDATA[Passianotto]]></surname>
<given-names><![CDATA[ALdL]]></given-names>
</name>
<name>
<surname><![CDATA[dos Santos]]></surname>
<given-names><![CDATA[JV]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Development of classification criteria for resistance to soybean rust and differences in virulence among Japanese and Brazilian rust populations]]></article-title>
<source><![CDATA[Tropical Plant Pathology]]></source>
<year>2010</year>
<volume>35</volume>
<numero>3</numero>
<issue>3</issue>
<page-range>153-62</page-range></nlm-citation>
</ref>
<ref id="B18">
<label>18</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[da Silva]]></surname>
<given-names><![CDATA[AF]]></given-names>
</name>
<name>
<surname><![CDATA[Sediyama]]></surname>
<given-names><![CDATA[T]]></given-names>
</name>
<name>
<surname><![CDATA[dos Santos Silva]]></surname>
<given-names><![CDATA[FC]]></given-names>
</name>
<name>
<surname><![CDATA[Bezerra]]></surname>
<given-names><![CDATA[ARG]]></given-names>
</name>
<name>
<surname><![CDATA[Rosa]]></surname>
<given-names><![CDATA[DP]]></given-names>
</name>
<name>
<surname><![CDATA[Cruz]]></surname>
<given-names><![CDATA[CD]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Effect of defoliation from the bottom to the top on the yield of Brazilian soybean cultivars]]></article-title>
<source><![CDATA[African Journal of Agricultural Research]]></source>
<year>2015</year>
<volume>10</volume>
<numero>33</numero>
<issue>33</issue>
<page-range>3296-304</page-range></nlm-citation>
</ref>
</ref-list>
</back>
</article>
