Abstract

RUIZ GARCIA, Relman et al. Evaluation of the cytotoxicity of arsenic trioxide in human tumoral cellular lines by the sulforhodamine B colorimetric technique. Rev cubana med [online]. 2006, vol.45, n.1, pp. 0-0. ISSN 1561-302X.

Arsenic trioxide has proved to be an inductor of apoptosis in cellular lines of acute promyelocytic leukemia. Clinical studies have showed its effectiveness in the treatment of this disease in patients refractory to the treatment with retinoic acid. It was determined the cytotoxic activity of this product against a panel of 3 human tumoral cellular lines of solid tumors (M14, MCF7, HT-29) and a line of acute promyelocytic leukemia, using the sulforhodamine B technique. Two experiments were made in which the cells were incubated for 48 hours in the presence of the product. The IC50 s obtained in both experiments were: M14 (0.471 and 0.450 µg/mL), MCF7 (0.122 µg/mL), HT-29 (0.543 and 0.659 µg/mL) and HL60 (0.487 and 0.351 µg/mL). It was concluded that the arsenic trioxide has a marked cytotoxic activity not only in the line of acute promyelocytic leukemia, but also in the studied lines of human solid tumors. Therefore, it was recommended the conduction of a study of the antitumoral activity of this product.

Keywords : Sulforhodamine B; cytotoxicity; human tumoral cellular lines; breast adenocarcinoma (MCF7); melanoma (M14); colon adenocarcinoma (HT29); acute promyelocytic leukemia.