Abstract

GANGES, Llilianne et al. ANTIGENIC, BIOLOGICAL AND MOLECULAR CHARACTERIZATION OF THE CUBAN CSFV ISOLATE "MARGARITA". Rev Salud Anim. [online]. 2007, vol.29, n.3, pp. 182-192. ISSN 0253-570X.

"Margarita" CSF virus isolate (Cuba, 1958) was characterized from the antigenic, biological and molecular point of view; adapted on PK-15 and SK-6 cell lines with an infective titer of 10^7.5 DICT₅₀,  and biologically cloned by limit dilution keeping its pathogenicity in pigs. Genotyping of this virus was made and its phylogenetic relationship with another strain deposited at the GenBank was determined keeping located in the 1.2 sub-group very close related with the field isolates from the 1993-2006 epidemic in Cuba. Its antigenic behavior was similar to the "Alfort" strain, showing no reactivity to MAbss against vaccine strains. The complete sequence of the gene for the E2 protein deposited in the EMBL with number AJ704817 is shown, also the a.a. deduced sequence showing all the residues of  cysteine highly conserved in the pestivirus genome is given being part of the different types of conformational epitopes  of the glycoprotein E2. From these results, an isolate of a Cuban-native CSF virus fully characterized is available, which can be used as reference material for several purposes.

Keywords : CSF; CSF reference strain; antigenic biological and molecular characterization.

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