Abstract

HERNANDEZ FORTE, Ionel et al. SELECTION OF RHIZOBIA ISOLATES FROM FORAGE LEGUME´S NODULES Canavalia ensiformis. cultrop [online]. 2012, vol.33, n.3, pp. 27-33. ISSN 0258-5936.

The objective of this work was to carry out a selection of rhizobia isolates from forage legume´s nodules Canavalia ensiformis according to their characteristics and potentialities to develop nitrogen-ûxing root nodules in Macroptilium atropurpureum (siratro), as model plant, so that they can constitute promissory strains for biofertilizer elaboration that improve the establishment of the canavalia in the cattle floors. Twelve rhizobia isolates and two commercial strains, BR 2001 and BR 2003a were used. The possible taxonomic distribution of the isolates was determinated throught the study of their morpho-cultural and physiological characteristics. In vitro nodulation assays were also performed in Macroptilium atropurpureum (siratro). Five possible members of the genera Rhizobium /Sinorhizobium and seven possible members of genera Bradyrhizobium were identified. Although the plants inoculated with the commercial strain BR 2003a presented a bigger number of total nodules and total effective nodules, the plants inoculated with the isolate CP5 presented a bigger number of nodules in the main root and those inoculated with CP12 had a bigger number of effective nodules in this root. The carried out study demonstrates that isolates of autochthonous rizobia as CP5 and CP12 they could constitute promissory strains for the biofertilizer elaboration that improves the establishment of the cultivation of canavalia in the cattle floors.

Keywords : Canavalia ensiformis; forage legumes; selection.

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