Abstract

VILASECA, JUAN CARLOS et al. Evaluación de un conjugado anti-IgG de ratón-fluoresceína mediante técnicas de inmunofluorescencia indirecta y citometría de flujo. Rev Cubana Med Trop [online]. 1997, vol.49, n.2, pp. 120-124. ISSN 1561-3054.

An immunoglobulin G of mouse was purified from sera by affinity chromatography in protein A. The rabbits whose sera were able to recognize the antigen injected by double immunodiffusion were immunized with this preparation. The antibodies were precipatated from the rabbit's serum and purified by ion exchange chromatography. This preparation was conjugated to fluorescin isothiocyanate according to the conventional technique. The conjugated obtained was evaluated with the reference strains of Parainfluenza virus 1, 2, 3; Adenovirus; respiratory syncytial virus; and influenza virus A and B, by an indirect immunofluorescence technique and HIV positive samples by flow citometry. Specific monoclonal antibodies were used in both cases. Clinical specimens of patients with acute respiratory infection were evaluated.

Keywords : IGG [isolation & purification]; FLOW CITOMETRY [methods]; FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT [methods]; MICE [blood]; CHROMATOGRAPHY, AFFINITY; CHROMATOGRAPHY; AFFINITY PUNCTURES; STAPHYLOCOCCAL PROTEIN A.