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Revista Cubana de Investigaciones Biomédicas
On-line version ISSN 1561-3011
Abstract
CABRERA LLANO, Jorge; CASTELLANOS GONZALEZ, Maricel and HIDALGO CALCINES, Pedro. C. Separación de hemoglobina A2 y hemoglobina C por cromatografía en carboximetilcelulosa (CM-52) con amortiguador imidazol-HCl-KCN-NaCl. Rev Cubana Invest Bioméd [online]. 1998, vol.17, n.3, pp. 234-239. ISSN 1561-3011.
A new macrochromatographic technique was assayed in carboxymethylcellulose and damperer iminazole-HCL-KCN-NaCL at 6,7 pH. This technique es reliable and exact for A2 hemoglobin separation and quantization in individuals carrier of AC hemoglobin, C Beta-thalasseamia, AS hemoglobin, and SC hemoglobin, whick, in many cases es of clinical interest. Also, a variant of A2 hemoglobin was detected in a pregnant woman carrier of AC hemoglobin, and a Beta-thalassemic gen in Cis with the gen for C hemoglobin.
Keywords : CHROMATOGRAPHY [methods]; HEMOGLOBIN A2 [analysis]; HEMOGLOBIN C [analysis].