Abstract

GONZALEZ-SIERRA, Liliet et al. Pytochemical characterization and total antioxidant activity of different extracts from Tithonia diversifolia (Hemsl) A. Gray. Pastos y Forrajes [online]. 2019, vol.42, n.3, pp. 243-248. ISSN 2078-8452.

In order to characterize, qualitatively and quantitatively, the main metabolites present in leaf, stem and root extracts from Tithonia diversifolia (Hemsl) A. Gray and determine the total antioxidant capacity of the different plant parts, the plant material was randomly collected in a farm belonging to a farmer of Matanzas province, Cuba. The leaf, stem and root samples were dried, pulverized and extracted with commercial ethanol by maceration. Afterwards, the solvent was removed through a vacuum rotary evaporator. The phytochemical sieving of the different extracts was carried out for the qualitative determination of total phenols, flavonoids, saponins, coumarin, tannins, quinones and terpenoids. The concentrations of total phenols, flavonoids and saponins of the three extracts were determined using a gallic acid pattern, quercetin and 10 % panax ginseng, respectively. The total antioxidant activity was measured through the phosphomolybdate method. The quantification was carried out in triplicate in 96-well microplates. The data were analyzed through descriptive statistics with the package SPSS®, Version 15. The results showed presence of phenols, flavonoids, saponins, coumarins, quinones and terpenoids in ethanol extracts of T. diversifolia roots, stems and leaves. The antioxidant activity showed that the root was the organ with higher antioxidant capacity, with 1,10 mg of ascorbic acid/mg of extract. It was followed by the leaves (1,08) and finally, the stem (0,50). The qualitative and quantitative determination of secondary metabolites showed the presence of phenols, flavonoids, coumarins, quinones and terpenoids in ethanol extracts of T. diversifolia roots, stems and leaves.

Keywords : leaves; metabolites; roots; stem.

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