Abstract

BERNAL, A; MARTINEZ, B  and  INFANTE, Danay. METHOD FOR THE EVALUATION OF THE RESPONSE OF TOMATO GENOTYPES INOCULATED WITH PASSALORA FULVA (COOKE) U. BRAUN AND CROUS IN A GREENHOUSE. Rev. Protección Veg. [online]. 2009, vol.24, n.2, pp. 102-105. ISSN 2224-4697.

A method for evaluating the resistance to leaf mold on Solanum plants was developed using conidial suspensions of Passalora fulva. Moneymaker cultivar and four Near Isogenic Lines (NILs), Cf-Ecp1, Cf-Ecp2, Cf-Ecp4 and Cf-Ecp5 were tested in a greenhouse. The inoculum was adjusted to 10⁵ UFC.mL^-1 and applied to the lower leaves. The plants were daily evaluated after inoculation up to 21 days. A standardized scale of leaf symptoms ensured consistency between evaluators. The genotypes, except Cf-Ecp1, Cf-Ecp2, Cf-Ecp4 and Cf-Ecp5 NILs, showed a similar response to P. fulva in natural conditions. The resistance expressed in its NILs was characterized by a hypersensible reaction. Artificial inoculation of plants was an easy, rapid and practicable method to determine resistance to P. fulva. An inoculum adjusted to an appropriate concentration gave uniform symptoms on the inoculated leaves. The method used proved to be a promissory tool for the evaluation of tomato genotype response in breeding programmes.

Keywords : scale; symptoms; tomato; Passalora fulva; greenhouse.

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