Abstract

MORALES, Juan; ORLANDO, Acosta; TAMAYO, Pablo  and  PENARANDA, José. Characterization of Citrus tristeza virus isolates from Colombia. Rev. Protección Veg. [online]. 2013, vol.28, n.1, pp. 45-53. ISSN 1010-2752.

Isolates of Citrus tristeza virus around the world differ widely in their biological properties. These properties may depend on strain mixtures within individual trees. Monoclonal Antibody 13 (MCA13) is an antibody that detects almost all the severe strains causing Quick Decline and Stem Pitting CTV syndromes. The objectives of this work were: I) to determine the percentage of CTV isolates reactive to the MCA13 antibody in each Colombian region evaluated and II) to analyze the genetic diversity of isolates from these regions. In almost all the regions evaluated, CTV MCA13 reactive isolates were identified; however, only one was found in Mompox area. The coat protein gene was amplified by reverse transcription-polymerase chain reaction (RT-PCR) and analyzed by single strain conformational polymorphisms (SSCP) patterns. In this work, at least twelve different dsRNA electrophoretic profiles and seven SSCP different electrophoretic patterns were identified. At least two different isolates were shown to be a mixture of strains. Non reactive strains to MAC13 from the Mompox region could be the best candidates for a Tristeza disease cross-protection program.

Keywords : virus cross-protection; citrus tristeza disease; Citrus spp; RT-PCR; SSCP.

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