Abstract

DUENAS RIVADENEIRA, Alex Alberto; ALCIVAR CEDENO, Ulbio Eduardo; OLAZABAL MANSO, Ervelio  and  CORTES RODRIGUEZ, Remigio. Antioxidant effect of the Chuquiraga jussieui J. F. Gmel using the hemolysis test. Medicentro Electrónica [online]. 2014, vol.18, n.2, pp. 57-64. ISSN 1029-3043.

The evaluation of the hemolytic and antioxidant effect of the aqueous extract of C. jussieui was carried out using the hemolysis test with the human erythrocyte produced by the sodium dodecyl sulphate (SDS). Five concentrations of the extract (7, 5, 15, 30, 60 and 120 mg/L) were evaluated. The hemolytic effect produced by the dodecyl sulphate was validated in a solution that contains erythrocytes, SDS and phosphate-buffered saline (PBS), and the hemoglobin concentration was obtained in the suspension using ultraviolet- visible spectroscopy (UV-Vis), the one that was used to determine the antioxidant effect of the extract of C. jussieui. The average concentration of hemoglobin (CH50) of SDS obtained was of 35,35 mg/L; while for the extract of C. jussieui, the (CI50) was of 64,89 mg/L and the (CI90) was of 86,96 mg/L. These results suggest the possible use of the extract of this plant as an antioxidant.

Keywords : plant extracts; antioxidants; hemolysis; erythrocytes; hemoglobins.

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