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Revista Cubana de Estomatología

On-line version ISSN 1561-297X

Abstract

PONS PINILLOS, Zoraida  and  HERNANDEZ RODRIGUEZ, Nadia. Variations of alkaline phosphatase enzyme in the dental pulp. Rev Cubana Estomatol [online]. 2005, vol.42, n.2, pp. 0-0. ISSN 1561-297X.

In the last decades, numerous investigations have been made on the study of potential mechanisms involved in the development of dental caries and their prevention. However, in spite of the gradual reduction of dental caries in the population, a lot of patients need to have their dental caries treated and different materials are continuously used searching for one that before the aggression to the pulp helps it to give a biological response, conserving this way its integrity. That's why the activity of the alkaline phosphatase of the pulp in the caries process is important as a reaction to the calcium hydroxide that is constantly utilized in the teaching-health service network of the country. 50 monoradicular teeth with living pulp and with caries of second, third and fourth degree, and 50 sound teeth from patients of different ages were selected. The pulp of each tooth was extracted and impressions were made (3 per sample). One of them was processed to obtain morphological guidance and the other two to assess the activity of alkaline phosphatase. The cobalt calcium method and Gomori's alpha naphthol phosphate method were used to this end. As a result, it was proved that the pulp has a higher enzymatic activity in deep caries and that the age of the patient does not determine the increase or decrease of this activity.

Keywords : Alkaline phosphatase of the dental pulp; calcium hydroxide; enzymatic activity of the pulp.

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