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Revista Cubana de Farmacia
On-line version ISSN 1561-2988
Abstract
DIEGUEZ CASTRO, R. Rosario; MUNOZ TRUJILLO, Yanin and CASTINEIRA DIAZ, Mirta. Western Blot technique applied to VA MENCOG BC® antimeningococcal vaccine. Rev Cubana Farm [online]. 2009, vol.43, n.3, pp. 0-0. ISSN 1561-2988.
ABSTRACT Western Blot technique applied to VA MENGOC BC® antimeningococcal vaccine was developed and validated and produced in "Carlos J. Finlay" Institute to demonstrate the identity criterion. Using this technique it was possible to identify antigenic proteins type P1, P3, P5 70 and 80 K present in the vesicle of external membrane and final vaccine, thus, we used the antimeningococcal gamma. Parameters developed in validation of this technique included: specificity, detection limit, repetition, average accuracy, reproduction, and strength, identity technique fulfilled with abovementioned parameters, considering like validated
Keywords : Western Blot Technique; Western blot validation; identity; VA MENGOC BC® antimeningococcal vaccine.