Abstract

VALLE ALVAREZ, Gladys María et al. Infected wound model. Rev Cub Med Mil [online]. 2005, vol.34, n.2, pp. 0-0. ISSN 1561-3046.

A biological model was established to reproduce an infection in surgical wounds due to Staphylococcus aureus in 10 male Hartley Guinea pigs with a variable weight between 269 and 320 g. Curve flaps were made in the skin at each side of the median line of approximately 2 cm of diameter and a bacterial culture of Staphylococcus aureus from a clinical isolation was applied with a brush at a concentration of 2 x 109UFC. Next, the skin was sutured in its normal position and the signs of infection were evaluated during 72 hours. After that, the sacrifice was carried out and a sample of the infected tissue was taken for histopathological study. From the macroscopic point of view, edema, rubor, exudation and abscess were observed. On the microscopic study, it was found predominance of acute infiltrate from the epidermis and inferior dermis that in 30 % of the animals included the muscular dermis with presence of fibrin. In the microbiological study, Staphylococcus aureus was recovered with an antibiotype pattern similar to that of the innoculated strain. These results allow to conclude that the infection of surgical wounds was attained under specific working conditions, which coincides with what is reported at the international level. This model is an efficient aid in the therapeutical studies of the infections caused by this germ.

Keywords : Staphylococcus aureus; surgical wound; experimental model; Guinea pigs.