Abstract

SOSA, Iliana et al. RECOMBINANT HUMAN ERYTHROPOIETIN WITH LOW SIALIC ACID PATHWAY TO THE CENTRAL NERVOUS SISTEM BY INTRANASAL ROUTE IN Meriones unguiculatus AND NO HUMAN PRIMATE Macaca fascicularis MODELS. Rev Salud Anim. [online]. 2008, vol.30, n.1, pp. 39-44. ISSN 0253-570X.

Our propose was to demonstrate the brain access of the recombinant human erythropoietin, with low sialic acid content, in the Mongolian gerbil and Macaca fascicularis, as well as the safety of nasal administration in both species. Eighteen Mongolian gerbils received intranasally I¹²⁵ labelled erythropoietin. Olfactory bulb and cerebellum-associated radioactivity were measured by a gamma counter. In a second experiment, erythropoietin was intranasally administered to 10 monkeys. Erythropoietin content in serum and cerebrospinal fluid obtained by lumbar puncture was determined by an ELISA method. From the intranasal administered radioactivity, a 0,8% was detected in the olfactory bulb at 5 min. In M. fascicularis, a 0,15% of the dose was determined in the cerebrospinal fluid 15 min after the intranasal administration. Treatment related-changes in blood parameters in both species were not observed. Results suggest that nasal route may be an alternative, non invasive and sure mode of access to the brain for the erythropoietin, which can be used as neuroprotector in stroke, a current health problem nowadays.

Keywords : erythropoietin; intranasal route; Macaca fascicularis; kinetics; safety; Mongolian gerbil; Meriones unguiculatus.

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