Abstract

RODRIGUEZ YON, JYakelín et al. Alternative stainning technique to determine mycorrhizal colonization. cultrop [online]. 2015, vol.36, n.2, pp. 18-21. ISSN 1819-4087.

The root stainning technique is neccesary in the work with arbuscular mycorrhizal fungi (AMF), which constitute the base of several Biofertilizers, as the EcoMic^® product, each time more used on agricultural practices at world level. That`s why the sample number evaluated at Mycorrhiza laboratories from diverse Research, Academic and even some productive Centers, increases every year. Those works require the application of such technique; nevertheless, it has the inconvenient to use highly toxic and carcinogenic reagents. The objective of the present work was to substitute those reagents by others non harmful to human health and environment. This is the case of Trypan blue that was substituted by the washable pen ink Parker QuinK. Moreover, Lactic acid and Glycerin were eliminated from the protocol. Results showed the pertinence of such modifications and revealed the usefulness and high quality of mentioned ink, which successfully stains all fungal structures allowing a clear visualization during long time

Keywords : arbuscular mycorrhiza; roots; colorants; health protection.

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