Abstract

PEREZ-PEREZ, Jorge L et al. Determination of minimum lethal concentration of Glufosinate-ammonium for selection of transformed somatic embryos of soybean cultivar INCASoy-27. cultrop [online]. 2015, vol.36, n.3, pp. 58-63. ISSN 1819-4087.

The establishment of a genetic transformation program requires a selection in vitro system of transformed tissues. In the Glycine genus exists few references that employ the Glufosinate-ammoniun that selective agent in transformed somatic embryos. For this reason this work had as objective to determine the minimum lethalconcentration of the herbicide Glufosinate-ammoniun in somatic embryos of Cuban soybean cultivar INCASoy-27, to use as selective agent in genetic transformation programs. The soybean somatic embryos were placed on multiplication medium that contained 2,4-dichlorophenoxyacetic acid 20 mg L^-1 with different concentrations of Glufosinate-ammonium (1,0; 2,0; 3,0; 4,0; 5,0; 6,0 mg L^-1). All concentrations of selective agent caused necrosis in not transformed soybean somatic embryos. The increments of selective agent concentration and culture time increased the necrosis of somatic embryos. Finally was determined the minimum lethal concentration of 6,0 mg L^-1 Glufosinate-ammoniun in semisolid medium, that selective agent in the genetic transformation of somatic embryos of soybean cultivar INCASoy-27

Keywords : agents; somatic embryogenesis; phosphinothricin; Glycine max; genetic transformation.

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