Abstract

LARA RODRIGUEZ, Regla M; FLORIDO BACALLAOL, Marilyn  and  VARELA NUALLES, Mario. Modification of the staining method of polyphenoloxidases and carbonic anhydrases in plants. cultrop [online]. 2016, vol.37, n.1, pp. 124-127. ISSN 1819-4087.

Isozymes have proved to be valuable in genetic diversity studies of different species. Therefore, this study was aimed to describe an efficient histochemical staining modification of carbonic anhydrase and polyphenol oxidase isozymes in plants, which consists of replacing the staining buffers used to develop these isozymes by distilled water. The effectiveness of these proposed adjustments was confirmed when using the “t” test of Student for related data and conducting these tests in different crops. The electrophoretic profiles observed in stainings showed the same pattern of bands without significant differences among treatments, demonstrating that it is possible to replace traditionally used staining buffers in these techniques by distilled water. The high repeatability of such stainings will enable to employ the proposed modifications to develop these isozymes

Keywords : biochemical markers; polymorphism; histochemical staining.

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