Abstract

POSADA-PEREZ, Laisyn et al. Efect of Pectimorf® on rooting and in vitro acclimatization of papaya (Carica papaya L.) shoots cultivar Maradol Roja. cultrop [online]. 2016, vol.37, n.3, pp. 50-59. ISSN 1819-4087.  http://dx.doi.org/10.13140/RG.2.1.1642.2642.

In vitro propagation of papaya has been successfully reported by different researchers, however the problems in the process of rooting and acclimatization have not been completely resolved yet. The low percentage of rooting and low survival of plants under conditions of acclimatization remain a problem for the development of an efficient micropropagation protocol for this crop. Employment growth regulator cuban Pectimorf® could be used to minimize this problem. In two experiments that were conducted as treatments are made up of two concentrations of sucrose (0 and 10 g L^-1), two of auxin AIB (0 and 2 mg L^-1) and five Pectimorf® concentrations (3, 5, 7, 9 and 12 mg L^-1). Were used as controls: the culture medium rooting composed of MS salts 50 %, 2 mg L^-1 IBA, 0,4 mg L^-1 thiamine, sucrose 40 g L^-1 agar 7 g L^-1 and the same culture medium without sucrose and Pectimorf®, but with zeolite as carrier. The results showed that the Pectimorf® had a positive effect on rooting and in vitro acclimatization of papaya shoot. The synergistic action of AIB with 9,0 mg L^-1 Pectimorf® allowed to obtain in vitro plants with greater leaf area, fresh weight, number of roots, photosynthetic rate and stomatal conductance, which together with a high percentage of rooting and less percentage of open stomata allowed to reach a 76,2 % survival ex vitro conditions

Keywords : auxin; oligosaccharides; papaya; roots; zeolite.

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