Abstract

RODRIGUEZ-YON, Yakelin; CHIRIBOGA-MOROCHO, Romel; CONCHA-EGAS, Elmo G.  and  LARA-RODRIGUEZ, Regla M.. Anhydrase carbonic activity in arbuscular mycorrhiza. cultrop [online]. 2016, vol.37, n.3, pp. 60-65. ISSN 1819-4087.  http://dx.doi.org/10.13140/RG.2.1.1511.1927.

The action of the metalloenzyme carbonic anhydrase (AC) in plants has been mainly associated to increase the CO₂ concentration inside chloroplast to enhance the carboxylation rate of Rubisco enzyme, being this reaction that integrates the CO₂ in carbohydrates during photosynthesis. However, the AC activity has been found in non-green tissues also, like roots, nodules, etiolated leaves, seeds, as well as in bacteria and fungi, which plays diverse and specific rolls. In the present study, the isoenzyme patterns of AC were analyzed in tomato (Solanum lycopersicum L.) roots inoculated in individual way with six different arbuscular mycorrhizal fungi (AMF) strains, taking the samples at two moments of symbiosis, and afterward they were compared among them. Results revealed that the AM fungus presence provoked the appearance of isoenzymes with AC activity in tomato roots cv. ‘AMALIA’, and the obtained isoenzymatic patterns showed a differential response dependent on inoculated strain, principally at early stages of symbiosis. The possible fungal origin of observed isoenzymes is discussed. The present work constitutes the first report about AC isoenzymes in AMF- colonized roots

Keywords : carbonic anhydrase; isoenzymes; mycorrhizae; tomato.

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