Abstract

FERNANDEZ SUAREZ, Kalyanne; DECLERCK, Stéphane; FERNANDEZ GARCIA, Loiret  and  ORTEGA DELGADO, Eduardo. Application of Mycelium Donor Plant (MDP) system on in vitro mycorrhization of potato. cultrop [online]. 2017, vol.38, n.1, pp. 31-38. ISSN 1819-4087.

In vitro mycorrhization of plants is a highly complex process that depends on ensuring the needs of plants and fungi, organisms with different nutritional requirements. Today doesn´t exist an in vitro culture system which guarantee the mycorrhization of Solanum tuberosum L. (potato), although some authors have made several attempts to achieve it. The aim of this study was to evaluate the applicability of Mycelium Donor Plant (MDP) system for in vitro mycorrhization of potato plants in order to get mycorrhized plants in a short period, with proven practical use. Experimentally, a similar system to MDP, in which S. tuberosum plants (seven days old) were associated with an extensive network of mycelium coming from Medicago truncatula plants, previously mycorrhized with the AMF Rhizoglomus intraradices was design. High levels of potato plant colonization (55 %), just 12 days after contact the mycelium networks, were obtained. Potato plants were in excellent conditions to be transplanted and they were able to reproduce the fungal colony when they were replanted in fresh media, producing a large number of extraradicals structures (mycelium and spores), demonstrating the applicability of MDP system for in vitro mycorrhization of S. tuberosum

Keywords : extraradical micelium network; in vitro culture; Rhizoglomus intraradices; Solanum tuberosum.

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