Abstract

HERNANDEZ FORTE, Ionel  and  NAPOLES GARCIA, María Caridad. Residents rhizobia in the rhizosphere of rice plants (Oryza sativa L.) cultivar inca lp-5. cultrop [online]. 2017, vol.38, n.1, pp. 39-49. ISSN 1819-4087.

The objective of this work was to isolate rhizobia from the rhizosphere of rice plants cultivar INCA LP-5. Rhizobia isolation from rhizospheric soil and rhizoplane of rice plants growing in Petroferric Ferruginous Nodular Gley soil was conducted. The possible taxonomic distribution of the isolates was determinated throught the study of their morpho-cultural and physiological characteristics. In vitro nodulation assays were also performed in Macroptilium atropurpureum (DC.) Urb. (siratro). Fourteen isolates showed similar cultural and morphological characteristics to rhizobia, of which 28,57 % were obtained from the rhizospheric soil and 71,43 % from rhizoplane of rice plants. All isolates were negative to cetolactase test and produced acid. An isolated showed moderate growth on the culture medium while the rest grew faster. Eleven rhizospherics isolated from rice plants produced effective nodules in siratro plants. A possible member of the Mesorhizobium genera and ten possible members of Rhizobiaceae family were identified. Four isolates showed a high effectiveness of the nodulation process in siratro plants and one of them significantly increased the root dry mass. The results obtained in this study provide the first evidence in Cuba where interaction of rhizobia-rice is developed

Keywords : isolation; Rhizobiaceae; rizobacteria.

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