Abstract

COSTALES, Daimy et al. Chitosans influence on soybean (Glycine max L. Merrill) nodulation and vegetative growth. cultrop [online]. 2017, vol.38, n.1, pp. 138-146. ISSN 1819-4087.

The purpose of this work was to study the in vitro compatibility of an induced inoculant containing of the Bradyrhizobium japonicum E109strain with chitosans of different physico-chemical characteristics. The influence of chitosan in vivo nodulation and growth of soybean plants, when applied to seeds at planting time (A), by foliar spraying to 15 days after seedling (B) and with the combination of both forms of application (C) was also evaluated. The chitosan and inoculant combination caused a variable effect in the number of viable bacteria until the thirty days of their conservation which depended on the chitosan type, the concentration, and the evaluation time. The response in plants was influenced by the application form and concentrations of chitosan assayed, but not for the differences between them in terms of molecular mass. The 100 and 500 mg L^-1 polymer and hydrolyzed chitosan concentrations, applied by foliar spray, exerted beneficial effect on increased number and nodule dry mass formed in soybean roots, respectively. Also, the root length was stimulated with different concentrations of partially hydrolyzed chitosan, applied by foliar spray. Our results suggest that the combination of chitosan with rhizobia inoculants can be used as organic fertilizers to enhance of soybean nodulation and growth and must be examined in future work, the application of chitosan in the field using the application forms studied

Keywords : Bradyrhizobium; leguminous; polymer; symbiosis.

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