Abstract

MARTINEZ-PACHECO, Javier; CASTRO-FERNANDEZ, María del Carmen; GONZALEZ-TOLEDO, Angélica  and  TOLEDO-SAMPEDRO, Verónica. The Php gene is not a resistance source in cuban tobacco. cultrop [online]. 2019, vol.40, n.1 ISSN 1819-4087.

The “black shank” is one of the main diseases that affect the commercial plantations of tobacco in the world. The identification of tobacco genes involved in resistance to the disease, as well as its introgression in commercial cultivars, constitutes a major challenge at present for the genetic improvement programs in Cuba. The information on the "pedigree" of the Cuban cultivars is scarce and sometimes erroneous, so it is important to determine with molecular tools the sources of resistance to the black shank in Cuba's commercial tobacco. The aim of this work was to determine the presence of the Php gene, as a source of monogenic resistance to the disease in 29 tobacco cultivars through the use of a specific molecular marker. It was shown that the Php gene was absent in all cultivars and that it has not been introduced through improvement programs for years. It is important to introduce the Php gene in new cultivars of Cuban tobacco through gene-pyramiding schemes that allow their combination with other sources of polygenic resistance to make the resistance more durable and having a wide spectrum.

Keywords : molecular marker; Nicotiana plumbaginifolia; cultivars; genetic breeding.

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