Abstract

NAPOLES-GARCIA, María Caridad et al. Induction of signals in Rhizobium leguminosarum bv. viciae and its biological activity in pea (Pisum sativum L.). cultrop [online]. 2021, vol.42, n.3  Epub Sep 30, 2021. ISSN 1819-4087.

Pea is a versatile plant, used in different ways and for different purposes, due to its high nutritional value. Its symbiotic interaction with bacteria of the rhizobia family guarantees significant amounts of nitrogen, from biological fixation, which allows it to grow in environments lacking this element. The agronomic and environmental importance of this symbiosis has required technology improvement for the production and inoculant application, so this work set out to evaluate the induction effect of a Rhizobium leguminosarum bv. viciae strain in the generation of signals in the inoculant and its biological activity on pea plants. Daidzein was used as an inducer at a final concentration of 5 µM. The lipidic fraction in the inocula was extracted with n-butanol and analyzed by high performance liquid chromatography and gas chromatography coupled to a mass spectrometer. Biological activity was evaluated in one experiment under controlled conditions, two field experiments, and one extensive trial. The results showed that in the inoculant induced with the flavonoid, a higher quantity and diversity of molecules related to the symbiotic interaction was detected. Similarly, the induced inocula showed a higher positive effect on nodulation (14.8 vs 8.7) and the yield of pea plants, with 468 and 125 kg ha-1 of seeds above the inoculated treatment without inducing in the experiments in plots and 746 kg ha-1 in the extensive trial.

Keywords : biological fixation; nodulation; communication; legumes.

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