Abstract

FRAGA NODARSE, Jorge et al. Comparison among 5 methods for the extraction of Triatominae DNA: its use in the random amplified polymorphic DNA technique. Rev Cubana Med Trop [online]. 2004, vol.56, n.3, pp. 203-207. ISSN 1561-3054.

The applicability of 5 protocols useful for the extraction of genomic DNA of triatominae was evaluated and the modified potassium acetate method was described as a method with which a high yield and purity of DNA is obtained in the shortest time and at the lowest cost to be used as a mold in the random amplified polymorphic DNA technique (simple method to detect the DNA genetic polymorphism) and probably in other molecular techniqeus based in the PCR amplification. The DNA quality is a crucial element for this technique, which needs a DNA extraction method as standardized as possible to obtain a pure non degraded DNA free of RNA and of PCR inhibitors, because changes in the purity can affect the amplification profiles and this is manifested in the presence of false bands and in the little reproducibility of the assay.

Keywords : RAPD; DNA extraction; Triatominae.

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