Abstract

DIAZ CONCEPCION, Alina et al. Platelet aggregating effect of 2 cytolysins: StI and StII purified from the sea anemone Stichodactyla helianthus. Rev Cubana Hematol Inmunol Hemoter [online]. 1999, vol.15, n.3, pp. 182-189. ISSN 1561-2996.

Cytolysing Sticholysina I (St I) and Sticholysina II (St II) induce platelet aggregation in the plasma rich in platelets in the range of assayed concentrations (0.5 to 10 m g/mL). Percentages of platelet aggregation over 90 % with less than 50 % of cellular lysis were obtained for both cytolysins. The platelet aggregation is high even when the cellular lysis decreases to less than 20 %. EDTA 2 mM/L and verapamyl 100 mM/L significantly inhibit the aggregation induced by StI, which shows that extracellular calcium has an important function in this process and that probably this cytolysin plays a role similar to that of a calcium ionophore. With StII there was no significant inhibition of the aggregation in the presence of EDTA and verapamyl. The aggregation induced by both cytolysins is not influenced by the increase of intracellular AMPc and it is independent of the formation of thromboxane A2 in the platelet

Keywords : CITOTOXINS [isolation & purification]; CHROMATOGRAPHY, GEL [methods]; CHROMATOGRAPHY, ION EXCHANGE [methods]; SEA ANEMONES; PLATELET AGGREGATION.

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