Abstract

MERLIN LINARES, Julio C et al. Preparation of a peroxidase-anti human IgG conjugate (chain g) in rabbit. Rev Cubana Hematol Inmunol Hemoter [online]. 2001, vol.17, n.2, pp. 132-137. ISSN 1561-2996.

A peroxidase conjugate was prepared starting from the specific antibodies isolated from an anti-chain rabbit serum and from human IgG. The specific antibodies were isolated by affinity chromatography and the conjugate was prepared by the method of oxidation with periodate. The conjugate obtained presented a molar IgG/peroxidase relation of 1.07, a RZ value of 0.33 and it was satisfactorily evaluated as regards its specificity and reactivity in the immunoenzimatic assays carried out

Keywords : ANTIBODIES; CHROMATOGRAPHY, AFFINITY; PEROXIDASES; IGG; RABBITS.

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