Abstract

RODRIGUEZ LEYVA, Rodisnel; MENENDEZ CORONA, Jesús; SILVEIRA SANCHEZ, Eglis  and  PANEQUE TAMAYO, Dalgis. Coombs serum production in sheep immunized with human globulin opsonized. Rev Cubana Hematol Inmunol Hemoter [online]. 2010, vol.26, n.3, pp. 228-235. ISSN 1561-2996.

An antiglobulin serum (Coombs) was obtained using a consistent inoculums in a immunocomplex (IC) the human immunoglobulin (Ig)/human antiglobulin in the seep by example, the opsonin to favor the immune response. Eighteen sheeps were immunized divided into three groups of 6 each: The first and second aimed to produce anti-IgG and anti-C3, respectively. In turn, these were divided into the A subgroup: in which we used the traditional method of Coombs's serum obtaining and B group in which we used the Freud's whole adjuvant in initial dose and the IC in the maintaining phase. Third group received the pure IC (A subgroup) and at a dilution of 1:200 (B subgroup). In sheeps from the 1B and 2B subgroups it was possible to obtain higher titration of anti-IgG and anti-C3dg than those immunized by means of the traditional method. The antibody response in animals immunized with the ICs (3A and 3B) was faster and of higher titration than those obtained by traditional method (1A and 2A) or the combined method (1B and 2B). The response in the 3B subgroup was lengthier apparently by a dose effect.

Keywords : Antiglobulin serum; immunization; immunocomplex; opsonin; Coombs's serum; Freud's whole adjuvant.

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