My SciELO
Services on Demand
Article
Indicators
- Cited by SciELO
Related links
- Similars in SciELO
Share
Revista Cubana de Hematología, Inmunología y Hemoterapia
On-line version ISSN 1561-2996
Abstract
SUAREZ REYES, Anamary et al. Association of peripheral blood lymphoid immunophenotype with presence of non-Hodgkin lymphomas. Rev Cubana Hematol Inmunol Hemoter [online]. 2022, vol.38, n.2 Epub June 01, 2022. ISSN 1561-2996.
Introduction:
Lymphocyte immunophenotype changes in non-Hodgkin lymphoma patients are associated with prognosis and therapeutic responses. However, its association with the disease has not been systematically established. Therefor its contribution to the diagnosis process.
Objective:
To assess the association of lymphocyte immunophenotype in peripheral blood with the presence of non-Hodgkin lymphoma.
Methods:
31 peripheral blood samples were analyzed from patients with a confirmed diagnosis of non-Hodgkin lymphoma and from 68 healthy individuals as controls, during the period 2018 to 2020. Multiparametric flow cytometry was used for immunophenotyping. The area under the curve and the Youden index were calculated to establish cut-off points in lymphocyte percentages. The association of immunophenotypic changes with non-Hodgkin's lymphoma was made using Odd ratio calculations.
Results:
The increase in TCD8+ and NKCD56dim lymphocytes from peripheral blood was significantly associated with the presence of non-Hodgkin lymphoma (OR= 3.4 and 2.9, respectively). Oppositely, the decrease in TCD4+, double positive T, double negative T and NKCD56bright lymphocytes was associated with the existence of non-Hodgkin lymphoma (OR= 23.0, 10.7, 6.9 and 15.8, respectively). Therefore, the decrease in the CD4/CD8 rate was also associated with the disease.
Conclusion:
The changes found in these lymphocytic immunophenotypes were significantly associated with the presence of non-Hodgkin lymphoma, which represents a systemic expression of the disease and suggests its diagnostic value.
Keywords : lymphoid immunophenotype; flow cytometry; peripheral blood; non-Hodgkin Lymphoma; diagnostic values.