Abstract

CAPIRO, Nancy et al. Capacidad protectora de Cymbopogon citratus (DC.) Stapf: ante el daño genético inducido por estrés oxidativo. Rev Cubana Invest Bioméd [online]. 2001, vol.20, n.1, pp. 34-37. ISSN 1561-3011.

The protective effect of a decoction of Cymbopogon citratus (DC) Stapf. against genetic damage induced by oxidizing agents H2O2 and ethylnitrosourea was studied. To this end, was used an experimental model in vitro and another in vivo; Chinese Hamster ovary cell culture and Drosophila melanogarster respectively. The colony-forming capacity of CHO cell line was the toxicity indicator used to evaluate 5 Cymbopogon citratus (DC) Stap. decoction concentrations (0,1; 1; 5; 10; 15 %) whereas the percentage of surviving adult insects was used to asses the toxic effect of 4 concentrations of this extract (5; 25; 50; 100 %) on mwh + 1 + flr³ larvae of Drosophila melanogaster. In both cases, the results revealed concentration dependent toxic effects. The cytofluourimetric studies performed on CHO cells with dischlorofluorescein-diacetate showed the antioxidant capacity of the decoction. Non-toxic doses of 0,1; 1 and 5 % caused significant reductions of H2O2 induced oxidative stress. Genotoxicity and antigenotoxicity of the different decoction concentrations were evaluated by chromosomal aberration detection tests in CHO cell and the SMART of Drosophila melanogastes' wings. The results of the mutagenic activity were negative whereas those of the protective capacity of the tested concentrations confirmed the antioxidant capacity of this vegetal extract

Keywords : OXIDATIVE STRESS; DNA DAMAGE; ANTIOXIDANTS [therapeutic use]; PLANTS, MEDICINAL [therapeutic use]; MEDICINE; HERBAL; CRICETULUS [genetic]; DROSOPHILA MELANOGASTER [genetic]; MODELS, BIOLOGICAL.

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