Abstract

SORIA PENALVER, Nelsa Esperanza; MORRIS QUEVEDO, Humberto Joaquín; RIVERA INFANTE, Sandra  and  GUTIERREZ CALZADO, Esteban. Obtainment and evaluation of the murine ascitic liquid anti-B hemo-classificatory in Ofbalb (F₁) and Balb/c mice strains. Rev Cubana Invest Bioméd [online]. 2010, vol.29, n.4, pp. 454-462. ISSN 1561-3011.

The Balb/c mouse strain has been traditionally used for the production of murine ascitic fluid; however, nowadays hybrid strains are introduced including the Ofbalb (F1). The aim of present paper was to assess the behavior of C6G4 (anti-B) hybridoma scattered in mice of Balb/c strain and in the Ofbalb (F1) hybrid strain. From each strain four groups were created which received by intraperitoneal route the cell concentrations of the inoculum: 1; 2,5; 3 and 4 x 10⁶ cells/0,5 mL/mouse. The analyzed parameters were the seizure percentage, the volume and the performance of murine ascitic fluid by mouse. It was demonstrated that with the Balb/c strain better results are obtained for this hybridoma in particular. The more appropriate inoculum was that corresponding to 2,5 x 106 cells. The Ofbalb (F1) hybrid strain with similar treatments not showed statistically significant differences in the analyzed parameters.

Keywords : Murine ascitic fluid; monoclonal antibodies; Ofbalb (F1) hybrid strain anti-B blood classifier..

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