SciELO - Scientific Electronic Library Online

 
vol.32 issue3Evaluation of the cytotoxicity of Clindamycin in cultures of gingival human fibroblastsCharacterization of the association between atherosclerosis and osteoporosis in men and women: study of deceased subjects author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand

Article

Indicators

  • Have no cited articlesCited by SciELO

Related links

  • Have no similar articlesSimilars in SciELO

Share


Revista Cubana de Investigaciones Biomédicas

Print version ISSN 0864-0300

Abstract

GARCIA HERNANDEZ, Yenela et al. Evaluation of a purified diet to obtain a biomodel of anemic rats. Rev Cubana Invest Bioméd [online]. 2013, vol.32, n.3, pp. 312-320. ISSN 0864-0300.

Introduction: the scientific and technological development of biological and biomedical sciences has been possible due to the use of experimental animal models. Iron- (Fe-) deficiency anemia affects about one third of the world population. Just weaned rats have been the animal model most commonly used to obtain an experimental biomodel of anemia. The disease is obtained by the hemoglobin (Hb) depletion method, feeding the animal an iron-deficient diet. Objective: evaluate the efficacy of a purified diet based on casein to obtain anemic rats. Method: a study was conducted of 40 just weaned (21-24 days) Sprague Dawley rats, 20 of each sex. The animals were housed in groups of 3 rats of the same sex in stainless steel boxes with mesh bottoms. For 8 weeks the animals were administered deionized water and a casein diet of 12.58% protein and 1.28 mgFe/kg. Body weight was measured every two weeks. Hb concentration was gauged from the fourth week onwards. Results: a significant increase in body weight was obtained, with no differences between male and female rats. The criterion for anemia was at least a 30% decrease in Hb with respect to the average baseline value per group. The percentage of anemic animals at 8 weeks was higher for females (75%) than for males (60%). Conclusions: the diet used for the study made it possible to obtain female anemic rats with acceptable efficiency in relation to the percentage of anemic animals and the increase in body weight.

Keywords : biomodels; anemia; diet; anemic rats.

        · abstract in Spanish     · text in Spanish     · Spanish ( pdf )