Abstract

ALONSO GELI, Yamirka; DE LA CRUZ, Enrique Reynaldo; DUTOK SANCHEZ, Carlos M.  and  ALVAREZ GUERRA, Eloy D. Detection of the epidermal growth factor receptor by relaxometry of oral premalignant lesions. Rev Cubana Invest Bioméd [online]. 2014, vol.33, n.2, pp. 150-160. ISSN 0864-0300.

Objective: Detect the overexpression of the epidermal growth factor receptor in epithelial cells of premalignant lesions of the oral mucosa magnetically marked by relaxometry. Methods: Exfoliated oral mucosa cells from healthy and sick individuals were marked with the system biotinylated anti-EGF-R IgG / anti-biotin IgG conjugated with supermagnetic particles and measurements were taken of relaxation times T1 and T2. Results: In all the samples analyzed there was a decrease in the relaxation times (T1 and T2) of marked cells with respect to unmarked cells. Comparison of the T1 and T2 values of marked cells from the lesion with the values of healthy cells revealed a statistically significant decrease in relaxation time. Comparison of the relaxation times of healthy marked cells with those of cells from the various lesions revealed statistically significant differences in both T1 and T2 in cells from lichen planus and leukoplakias, but not in cells from keratoses. Conclusions: Results show that relaxometry may be used to detect cells expressing the epidermal growth factor receptor, using the conjugated system described herein, and that EGF-R is overexpressed in cells from lesions such as leukoplakia and lichen planus.

Keywords : epidermal growth factor receptor; premalignant lesions; proton magnetic relaxation; relaxation time.

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