Abstract

BECQUER, C. J; PREVOST, Danielle; GAUVIN, Carole  and  BEADOUIN, Annie. Symbiotic efficiency of native rhizobia from Sancti Spíritus, Cuba, inoculated in Centrosema molle. Pastos y Forrajes [online]. 2013, vol.36, n.3, pp. 322-328. ISSN 0864-0394.

An essay was conducted in greenhouse, in order to determine the symbiotic efficiency of 39 native strains of bradyrhizobia from Sancti Spíritus. The isolations were made from root nodules of Centrosema molle, Centrosema virginianum and Centrosema plumieri, which are macrosymbionts from the central and southern areas of Sancti Spíritus. The obtained strains were inoculated in C. molle, promising forage legume for livestock feeding in the territory. The inoculation methods were standard, according to the recommendations in the international literature. A randomized block experimental design was used, with 41 treatments and three replications. Variance analysis (ANOVA) was made and the dry weight of the aerial part of the plant, root dry weight, nodulation index and nitrogen yield were evaluated. It is concluded that there was high symbiotic efficiency of the native strains from the central area, by being inoculated in a legume from the same zone; while the ones from the southern area were inefficient in such legume. On the other hand, the strains JK3 (C. virginianum), HG1 (C. plumieri) both from the central area and SP20 (C. molle, southern zone) and JJ2 (C. virginianum, central zone), showed high efficiency levels in the evaluated legume, which makes them the most promising ones. The high nodulation indexes of JH4, JJ2, JJ7, JK6, JK1 and JK5 (C. virginianum) did not imply high symbiotic efficiency. A selection phase in the field is recommended, with different macrosymbionts and under stressful soil and climate conditions.

Keywords : Bradyrhizobium sp; Centrosema sp; inoculation.

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