Abstract

JACAS TORNE, Manuel Facundo; SANCHEZ NODA, Eddy Orestes; GARCIA MESA, Norberto Regino  and  PINA ARES, Deisys. Spongy bone tissue sterilized with ethylene oxide gas. Rev Cubana Ortop Traumatol [online]. 2012, vol.26, n.2, pp. 143-155. ISSN 0864-215X.

Objective: verify the sterilizing properties of ethylene oxide gas over the spongy bone used for implants in medical practice, mainly its efficacy against certain microorganisms and the time required for sterilization in industrial autoclaves. Methods: 54 cube-shaped spongy bone tissue samples were prepared and distributed into 3 groups, each with 18 samples. The first group was used to evaluate a bacteriological contamination method with strains of known microorganisms. Upon contamination with the known microorganisms, the second and third groups were subjected to the effect of ethylene oxide gas in subgroups of 6 samples during 60, 90 and 120 minutes. Results: in the first group 100 % of the samples contaminated were positive. In the second and third groups, the effectiveness of ethylene oxide gas was 100 % at 120 minutes. Conclusion: this is a safe method appropriate for use in medical practice.

Keywords : ethylene oxide gas; bone tissue; sterilization.

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