Abstract

MONTES DE OCA, Nivian et al. KLAMIC: TECHNICAL-PRODUCTIVE EXPERIENCE. Rev. Protección Veg. [online]. 2009, vol.24, n.1, pp. 62-65. ISSN 2224-4697.

The National Center of Animal and Plant Health has a technology for manufacturing an agriculture bionematicide known as KlamiC. It is obtained from the strain of the fungus SD 187 IMI Pochonia chlamydosporia var. catenulata (Kamyscho ex Barron and Onions) Zare and W. Gams, which is a biological control agent of root-knot nematodes. For five years, 98 batches of 25 kg at pilot scale have been produced, representing more than 1500 kg of product. The quality indicators of the product KlamiC have remained within the ranges established for the product with an average concentration of 1,6x10⁷ chlamydospore.g of substrate^-1, 91,32% viability of chlamydospores, 77,04% parasitism of M. incognita eggs, 5,43% water content and a concentration of micro-pollutants of 8,1x10⁴ CFU.g of product ^-1. The application of the fermentation technology in solid state in polypropylene bags under the fulfilment of good manufacturing practices has enabled the obtaining of a quality inoculum, mainly used in characterization studies for toxicological and ecotoxicological testing, formulation, as well as in applications in sheltered crops and intensive orchards to demonstrate the capabilities of the fungus to settle in the soil and reduce nematode populations.

Keywords : Pochonia chlamydosporia; Meloidogyne spp; bionematicide; manufacture of biopesticides; quality management.

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