Abstract

BARRAGAN, Carlos Eduardo  and  GUZMAN-BARNEY, Mónica. Molecular detection of Potato yellow vein virus in the natural whitefly vector Trialeurodes vaporariorum, Westwood. Rev. Protección Veg. [online]. 2014, vol.29, n.3, pp. 168-176. ISSN 1010-2752.

Potato yellow vein virus (PYVV) expresses yellowing symptoms on plants and it is transmitted by the whitefly vector Trialeurodes vaporariorum Westwood. No molecular viral detection from the vector extracts has been reported to date. The objectives of this study were I) amplification of the PYVV major coat protein (CP) gene from viruliferous vector extracts by RT-PCR using specific primers, II) cloning and III) sequencing and sequences analysis. Some Colombian Solanum phureja (Jus et Buk) field potato plants expressing yellowing symptoms and confirmed PYVV positive by RT-PCR were used as virus donor plants for feeding whiteflies. Viruliferous vectors were obtained by depositing aviruliferous whiteflies on symptomatic leaves and kept in a muslin cloth-covered glass flask for 48 h. A kit SV for total RNA Isolation System (Promega^®) was used for extracting the viral RNA from one viruliferous whitefly and from pools of viruliferous vectors (2, 5, 10, 15 and 20 individuals); CP gene amplicons of about 765bp were cloned and sequenced to confirm the PYVV genome. Blast alignment established more than 99% similarity between CP gene amplified from vector extracts and several GenBank reported PYVV CP genes from Colombian and Peruvian isolates.This study presents information concerning the molecular detection of PYVV in its natural vector for the first time by using a very simple viral acquisition technique for obtaining viruliferous vectors that could be of interest for future studies concerning virus and vector gene characterization, plant-virus and vector interaction and/or epidemiological studies.

Keywords : PYVV; RNA; whitefly; detection; vector.

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