Abstract

ADAY DIAZ, Osmany de la C. et al. Distribution of the Sugarcane yellow leaf virus in Cuba. Rev. Protección Veg. [online]. 2014, vol.29, n.3, pp. 177-184. ISSN 1010-2752.

The present study had the purpose of determining the distribution of the Sugarcane yellow leaf virus (SCYLV) and its genetic variants occurring in different provinces of the country. Thirty five fields were examined (348 ha), and 525 samples of leaves were taken in 13 commercial cultivars. Detection of the virus was carried out by Tissue Blot Immunoassay (TBIA) and the molecular analysis by Reverse Transcription- Polymerase Chain Reaction (RT-PCR). The positive samples were used to identify the genetic variants by RT-PCR with specific primers. In most of the provinces, the incidence of symptoms associated with this disease (82.29%) was high. This is the first time that this pathogen has been detected in commercial areas of all the provinces where sugarcane is cultivated in Cuba. In most of the infected cultivars, the highest severity grade of the symptoms associated with this disease was observed. The presence of three genetic variants of SCYLV was determined: BRA-PER, CUB and REU. Mixed infections of the genotypes, BRA-PER + CUB and BRA-PER + REU, were also observed.

Keywords : Sugarcane yellow leaf virus; SCYLV; geographical distribution; genetic variants.

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