Abstract

CORZO LOPEZ, Mylene; PLASENCIA MARQUEZ, Odaylin; MARTINEZ COCA, Benedicto  and  QUINONES PANTOJA, Madelaine L.. Molecular variability of Xanthomonas axonopodis pv. phaseoli isolates from Mayabeque Province, Cuba. Rev. Protección Veg. [online]. 2016, vol.31, n.1, pp. 70-76. ISSN 2224-4697.

The aim of this work was the molecular characterization by rep-PCR of isolates of Xanthomonas axonopodis pv. phaseoli from different common bean (Phaseolus vulgaris L.) producing areas in the province Mayabeque. Twenty-nine isolates were obtained from thirty symptomatic samples collected, to which the biochemical, physiological, morphological, and pathogenic characteristics were previously determined. A high genetic variability was detected among the analyzed isolates, which formed four major genetic groups with a coefficient of similarity of 73% between them and a high number of haplotypes or subgroups (22 haplotypes). The four gene groups were composed of isolates that caused different symptoms. No direct relationships were observed between this genetic variability among isolates and the pathogenic characteristics present under controlled conditions. The wide genetic variability that exists in isolates of Xanthomonas axonopodis pv phaseoli associated with different cultivars of common bean under field conditions was shown.

Keywords : rep-PCR; common bacteria bligth (CBB); Phaseolus vulgaris.

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