Abstract

SAMANIEGO-FERNANDEZ, Luz María et al. “Isolation, identification, and evaluation of indigenous strains of Trichoderma spp. as antagonistic fungi of soil pathogens”. Rev. Protección Veg. [online]. 2018, vol.33, n.3 ISSN 2224-4697.

A survey was carried out in two agroecosystems with a red ferralitic soil in the province of Matanzas, Cuba, to isolate, identify and evaluate autochthonous strains of Trichoderma spp. From only one of the ecosystems, three autochthonous strains of this fungal species were isolated and identified. The in vitro antagonism of the autochthonous species obtained and of the reference species Trichoderma harzianum Rifai 3 (A-34) 2 and Trichoderma viride Persoon 5 (TS-3) 1 against the phytopathogenic fungi Rhizoctonia solani Kühn, Fusarium spp., and Sclerotium rolfsii Sacc., causal agents of crop diseases in the province, was evaluated using the dual culture technique. The daily linear growth (colony radii ), growth rate and percentage of radial growth inhibition (PICR) of the antagonists were determined. Their values were processed by Simple Variance Analysis. Trichoderma autochthonous strains isolated and identified were Trichoderma 2T-isolate, T. harzianum 6 (A-53) 2 and T. viride 4 (TS-3) 1, of which, Trichoderma 2T-isolate was the most efficient in vitro antagonist against the three phytopathogenic fungi, followed by T. viride 4 (TS-3) 1 (biocontroller of R. solani and S. rolfsii and with less antagonistic capacity against Fusarium spp.) and T. harzianum 6 (A-53) 2. Among the reference strains, T. harzianum 3 (A-34) 2 presented the greatest antagonism against these fungi. Both autochthonous and reference strains showed different levels of in vitro antagonistic activity agains tthe phytopathogens.

Keywords : antagonism; competition; biological control; micoparasitism; Trichoderma.

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