SciELO - Scientific Electronic Library Online

 
vol.19 issue1Strategy to verify quality of Escherichia coli strains kept in the collection of the Center of Genetic Engineering and BiotechnologyEvaluation of the potential of Mycobacterium smegmatis as vaccine Candidate against tuberculosis by in silico and in vivo studies author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand

Article

Indicators

  • Have no cited articlesCited by SciELO

Related links

  • Have no similar articlesSimilars in SciELO

Share


Vaccimonitor

On-line version ISSN 1025-0298

Abstract

AGUILAR, Alicia et al. In vitro neutralization assay to evaluate new hepatitis A vaccine candidates. Vaccimonitor [online]. 2010, vol.19, n.1, pp. 16-19. ISSN 1025-0298.

Immunity to hepatitis A virus (HAV) is in first instance due to neutralizing antibodies. Neutralization assays are essential to evaluate new candidate vaccines against this pathogen. In this paper, an in vitro neutralization assay to evaluate HAV vaccine candidates using phage-display technology is described. 103 and 102 DICT50 of HAV were incubated with different antibodies preparations and were inoculated onto plates with FRhK4 cells. After seven days of incubation, the neutralizing titer was determined as reciprocal of the serum dilution reducing HAV growth (inhibition of CPE) by 50%. 103 and 102 DICT50 were neutralized by 7E7 anti-HAV monoclonal antibody and anti-VHA positive human sera (A12 and A31). Negative controls of the assay did not neutralize viral infectivity. Sera from mice immunized with phages displaying VHA mimotopes had neutralizing titers from 4-16. Neither negative control nor pre-immune and sera from mice immunized with M13 wild type phage neutralized HAV. A simple and faster in vitro neutralization assay was developed to evaluate new HAV vaccine candidates.

Keywords : HAV; neutralization; mimotope; immunogenicity; phages.

        · abstract in Spanish     · text in Spanish     · Spanish ( pdf )

 

Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License