Abstract

VALDES-HERNANDEZ, Iliana et al. Differences in phagocytosis of Mycobacterium ´habana´ TMC 5135 by murine macrophages cellular cultures. Vaccimonitor [online]. 2018, vol.27, n.1, pp. 1-7. ISSN 1025-0298.

Tuberculosis remains as a major problem in the global health. BCG is the available vaccine against tuberculosis but only protects against severe form of disease during childhood, so the search for new vaccine candidates is a challenge. Taking into account the protective capacity of Mycobacterium ´habana´ against experimental tuberculosis, we proposed in vitro experiments using murine macrophages (peritoneal macrophages and cell line Raw 264.7) to characterize phagocytic process of this candidate. Phagocitic index and phagocytic number were calculated. The present work demonstrated that the phagocytosis process of M. ‘habana’ TMC 5135 is influenced by the cellular source used as host cell, as well as by the infecting bacterial load and the time of exposure. The present investigation contributes to the characterization of the infection by this mycobacteria in its main target cells of innate immunity and it suggest future investigations to evaluate the activation of effector mechanisms of the innate immunity against this candidate

Keywords : Mycobacterium ´habana´; vaccine; tuberculosis; macrophages.

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