Abstract

MATOS-REINOSA, Daimar; CASTILLO-PUENTES, Idalmelis del; MOLINA-HERNANDEZ, Raychel  and  MELCHOR-RODRIGUEZ, Antonio. Establishment of more efficient conditions for the production of glucose biosensors. Vaccimonitor [online]. 2018, vol.27, n.1, pp. 22-27. ISSN 1025-0298.

Biosensors are mobile devices that allow rapid and easy detection of metabolic diseases and viral infections of veterinary and clinical interest, such as rotavirus and hepatitis B and C. The objective of this work was to determine the significant variables of the production process of the glucose biosensors manufactured in the Immunoassay Center (Havana, Cuba). Eight experimental runs were carried out taking into account the normative operating procedures of the biosensor production plant. Accuracy tests were carried out to release produced batches. The experiments provided information about which factors should be carefully controlled during the manufacture procedure in order to avoid high levels of faulty products or the erratic behavior of the process. The factors selected for the study were those related with the preparation of the enzymatic solution. A multiple regression analysis was carried out to determine the statistically significant factors of the model. The coefficient of determination was higher than 90%, and the 98.637% of the variation between the values of percentage of accuracy and the mean value could be explained. The significant factors were the concentration of the glucose oxidase enzyme, the electric mediator concentration, and the ultrapure water conductivity (95% confidence level). The analysis carried out showed satisfactory results. In the present study, it was demonstrated that varying parameters of the production process it is possible to decrease the accuracy percentage values

Keywords : Biosensors; glucose; glucose oxidase.

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