Abstract

GARCIA-SUAREZ, José; ZUMALACARREGUI-DE-CARDENAS, Lourdes  and  SANTANA-VAZQUEZ, Zeila. Uses of Pichia pastoris yeast in the production of recombinant proteins. Vaccimonitor [online]. 2021, vol.30, n.3, pp. 153-163.  Epub Dec 01, 2021. ISSN 1025-0298.

Pichia pastoris metylotrofic yeast (currently classified as Komagataella phaffii) is one of the most important yeast for the production of heterologous proteins. The work presents an analysis of the main characteristics that are marked in the production of recombinant proteins expressed in Pichia pastoris. It describes the strains available for the transformation and production of recombinant proteins expressed in P. pastoris, the main commercial vectors for expression, the most efficient promoters, selectable markers, the secretion signal, the methods used in genetic transformations and glycosylation patterns that occur. General recommendations are provided on bioprocess parameters such as media composition, pH, temperature, aeration velocity, induction, and feeding strategies to achieve high productivity values. The results of Pichia pastoris applications for the production of two vaccines in Cuba, the hepatitis B vaccine and the tick control vaccine are shown.

Keywords : vaccines; recombinant proteins, yeasts; protein engineering.

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