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Vaccimonitor
On-line version ISSN 1025-0298
Abstract
CARDOSO-SAN JORGE, Felix et al. Standardization of a spectrophotometric procedure for the quantification of Neisseria meningitidis serogroup X capsular polysaccharide. Vaccimonitor [online]. 2022, vol.31, n.2, pp. 83-89. Epub Aug 01, 2022. ISSN 1025-0298.
The present work comprises the standardization a spectrophotometric procedure for assessing Neisseria meningitidis, serogroup X capsular polysaccharide and their intermediates of modification, the phosphodiesters groups present in its structure, based on Chen method. An analysis of the following standardization criteria was performed: linearity, precision (repeatability and intermediate precision) and accuracy.
It was demonstrated through the experimental design and the statistical procedures used that the method is linear (r > 0.99), the coefficient of variation of the response factor < 5%, the relative standard deviation of the slope < 2%, with no statistically significant difference between the intercept of the equation with respect to zero; exact, because there is no statistically significant difference between the concentration determined in a work material and its nominal concentration; it also proved to be repeatable, because the coefficient of variation of the concentrations of the sample (2.44; 2.43; 0.88 % for low, medium and high concentrations respectively) is less than 3% and there is no statistically significant difference between the means of the results obtained by two analysts, evaluated for four days at three concentration levels. Its intermediate precision was satisfactory.
Keywords : Neisseria meningitides; phosphorus; standardization; spectrophotometry.